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Title: Apparatus for generating x-ray holograms

Abstract

Apparatus for x-ray microholography of living biological materials. A Fourier transform holographic configuration is described as being most suitable for the 3-dimensional recording of the physical characteristics of biological specimens. The use of a spherical scatterer as a reference and a charge-coupled device two-dimensional detector array placed in the forward direction relative to the incident x-radiation for viewing electromagnetic radiation simultaneously scattered from both the specimen and the reference scatterer permits the ready reconstruction of the details of the specimen from the fringe pattern detected by the charge-coupled device. For example, by using a nickel reference scatter at 4.5 nm, sufficient reference illumination is provided over a wide enough angle to allow similar resolution in both transverse and longitudinal directions. Both laser and synchrotron radiation sources are feasible for generating microholographs. Operation in the water window (2.4 to 4.5 nm) should provide maximum contrast for features of the specimen and spatial resolution on the order of the wavelength of x-radiation should be possible in all three dimensions, which is sufficient for the visualization of many biological features. It is anticipated that the present apparatus will find utility in other areas as well where microscopic physical details of a specimen aremore » important. A computational procedure which enables the holographic data collected by the detector to be used to correct for misalignments introduced by inexact knowledge of the relative positions of the spherical reference scatterer and the sample under investigation has been developed. If the correction is performed prior to reconstruction, full compensation can be achieved and a faithfully reconstructed image produced.« less

Inventors:
 [1];  [2];  [2];  [1]
  1. (Chicago, IL)
  2. (Los Alamos, NM)
Issue Date:
Research Org.:
MCR Technology Corporation (Chicago, IL)
OSTI Identifier:
867521
Patent Number(s):
4955974
Assignee:
MCR Technology Corporation (Chicago, IL) CHO
DOE Contract Number:  
FG02-86ER13610
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
apparatus; generating; x-ray; holograms; microholography; living; biological; materials; fourier; transform; holographic; configuration; described; suitable; 3-dimensional; recording; physical; characteristics; specimens; spherical; scatterer; reference; charge-coupled; device; two-dimensional; detector; array; placed; forward; direction; relative; incident; x-radiation; viewing; electromagnetic; radiation; simultaneously; scattered; specimen; permits; reconstruction; details; fringe; pattern; detected; example; nickel; scatter; nm; sufficient; illumination; provided; wide; angle; allow; similar; resolution; transverse; longitudinal; directions; laser; synchrotron; sources; feasible; microholographs; operation; water; window; provide; maximum; contrast; features; spatial; wavelength; dimensions; visualization; anticipated; utility; microscopic; computational; procedure; enables; data; collected; correct; misalignments; introduced; inexact; knowledge; positions; sample; investigation; developed; correction; performed; prior; compensation; achieved; faithfully; reconstructed; image; produced; relative position; biological specimens; charge-coupled device; physical characteristics; coupled device; detector array; electromagnetic radiation; radiation source; spatial resolution; fourier transform; provide maximum; relative positions; longitudinal direction; fringe pattern; synchrotron radiation; spherical reference; biological material; reconstructed image; radiation sources; performed prior; two-dimensional detector; direction relative; formed prior; biological materials; data collected; forward direction; physical characteristic; /378/359/

Citation Formats

Rhodes, Charles K., Boyer, Keith, Solem, Johndale C., and Haddad, Waleed S. Apparatus for generating x-ray holograms. United States: N. p., 1990. Web.
Rhodes, Charles K., Boyer, Keith, Solem, Johndale C., & Haddad, Waleed S. Apparatus for generating x-ray holograms. United States.
Rhodes, Charles K., Boyer, Keith, Solem, Johndale C., and Haddad, Waleed S. Mon . "Apparatus for generating x-ray holograms". United States. https://www.osti.gov/servlets/purl/867521.
@article{osti_867521,
title = {Apparatus for generating x-ray holograms},
author = {Rhodes, Charles K. and Boyer, Keith and Solem, Johndale C. and Haddad, Waleed S.},
abstractNote = {Apparatus for x-ray microholography of living biological materials. A Fourier transform holographic configuration is described as being most suitable for the 3-dimensional recording of the physical characteristics of biological specimens. The use of a spherical scatterer as a reference and a charge-coupled device two-dimensional detector array placed in the forward direction relative to the incident x-radiation for viewing electromagnetic radiation simultaneously scattered from both the specimen and the reference scatterer permits the ready reconstruction of the details of the specimen from the fringe pattern detected by the charge-coupled device. For example, by using a nickel reference scatter at 4.5 nm, sufficient reference illumination is provided over a wide enough angle to allow similar resolution in both transverse and longitudinal directions. Both laser and synchrotron radiation sources are feasible for generating microholographs. Operation in the water window (2.4 to 4.5 nm) should provide maximum contrast for features of the specimen and spatial resolution on the order of the wavelength of x-radiation should be possible in all three dimensions, which is sufficient for the visualization of many biological features. It is anticipated that the present apparatus will find utility in other areas as well where microscopic physical details of a specimen are important. A computational procedure which enables the holographic data collected by the detector to be used to correct for misalignments introduced by inexact knowledge of the relative positions of the spherical reference scatterer and the sample under investigation has been developed. If the correction is performed prior to reconstruction, full compensation can be achieved and a faithfully reconstructed image produced.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}

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