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Title: Absorption-emission optrode and methods of use thereof

Abstract

A method and apparatus for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance.

Inventors:
 [1]
  1. (Livermore, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
867412
Patent Number(s):
4929561
Assignee:
Regents of University of California (Oakland, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
absorption-emission; optrode; methods; method; apparatus; monitoring; physical; chemical; properties; sample; fluid; measuring; optical; signal; generated; fluorescent; substance; modulated; absorber; emission; band; overlaps; absorption; degree; overlap; dependent; immobilized; substrate; effective; molecules; sufficiently; close; resonant; energy; transfer; occur; providing; highly; efficient; modulation; emissions; absorption band; energy transfer; sample fluid; signal generated; highly efficient; optical signal; fluorescent emissions; chemical properties; fluorescent emission; /436/250/422/

Citation Formats

Hirschfeld, Tomas B. Absorption-emission optrode and methods of use thereof. United States: N. p., 1990. Web.
Hirschfeld, Tomas B. Absorption-emission optrode and methods of use thereof. United States.
Hirschfeld, Tomas B. Mon . "Absorption-emission optrode and methods of use thereof". United States. https://www.osti.gov/servlets/purl/867412.
@article{osti_867412,
title = {Absorption-emission optrode and methods of use thereof},
author = {Hirschfeld, Tomas B.},
abstractNote = {A method and apparatus for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}

Patent:

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