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Title: Nucleic acid isolation process

Abstract

A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain with the DNA. These products can be removed with organic extraction, but the process steps associated with organic extraction reduce the size of DNA fragments available for experimental use. The present process removes the waste products by dialyzing a solution containing the DNA against a solution containing polyethylene glycol (PEG). The waste products dialyze into the PEG leaving isolated DNA. The remaining DNA has been prepared with fragments containing more than 160 kb. The isolated DNA has been used in conventional protocols without affect on the protocol.

Inventors:
 [1];  [2];  [1]
  1. Los Alamos, NM
  2. La Jolla, CA
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
OSTI Identifier:
867364
Patent Number(s):
4921952
Assignee:
United States of America as represented by United States (Washington, DC)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; isolation; process; method; provided; isolating; dna; eukaryotic; cell; flow; sorted; chromosomes; removed; chromosome; structure; detergent; proteolytic; digestion; products; remain; organic; extraction; steps; associated; reduce; size; fragments; available; experimental; removes; waste; dialyzing; solution; containing; polyethylene; glycol; peg; dialyze; leaving; isolated; remaining; prepared; 160; kb; conventional; protocols; affect; protocol; waste product; cell structure; ethylene glycol; polyethylene glycol; isolated dna; solution containing; nucleic acid; dna fragments; waste products; dna fragment; organic extraction; containing poly; process removes; process step; flow sorted; process steps; acid isolation; /999/435/

Citation Formats

Longmire, Jonathan L, Lewis, Annette K, and Hildebrand, Carl E. Nucleic acid isolation process. United States: N. p., 1990. Web.
Longmire, Jonathan L, Lewis, Annette K, & Hildebrand, Carl E. Nucleic acid isolation process. United States.
Longmire, Jonathan L, Lewis, Annette K, and Hildebrand, Carl E. Mon . "Nucleic acid isolation process". United States. https://www.osti.gov/servlets/purl/867364.
@article{osti_867364,
title = {Nucleic acid isolation process},
author = {Longmire, Jonathan L and Lewis, Annette K and Hildebrand, Carl E},
abstractNote = {A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain with the DNA. These products can be removed with organic extraction, but the process steps associated with organic extraction reduce the size of DNA fragments available for experimental use. The present process removes the waste products by dialyzing a solution containing the DNA against a solution containing polyethylene glycol (PEG). The waste products dialyze into the PEG leaving isolated DNA. The remaining DNA has been prepared with fragments containing more than 160 kb. The isolated DNA has been used in conventional protocols without affect on the protocol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}

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