Combined electrophoresis-electrospray interface and method
Abstract
A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.
- Inventors:
-
- Richland, WA
- North Augusta, SC
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- OSTI Identifier:
- 867199
- Patent Number(s):
- 4885076
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- combined; electrophoresis-electrospray; interface; method; analyzing; molecular; constituents; composition; sample; forming; solution; separating; capillary; electrophoresis; eluent; longitudinally; separated; according; relative; electrophoretic; mobilities; electrospraying; form; charged; spray; temporal; distribution; detecting; collecting; accordance; high-voltage; 5-100; kvdc; applied; applying; voltage; 2-8; exit; cathode; spaced; front; complete; electrical; circuit; formed; conductor; directly; contacts; conduction; sheath; electrode; discharged; annular; flow; temporal distribution; molecular constituents; sheath flow; capillary electrophoresis; electrical circuit; capillary exit; relative electrophoretic; separated according; separated constituents; charged spray; combined electrophoresis-electrospray; composition sample; complete electrical; cathode spaced; analyzing molecular; electrophoresis-electrospray interface; electrophoretic mobilities; longitudinally separated; constituents longitudinally; directly contacts; /204/250/
Citation Formats
Smith, Richard P, Udseth, Harold R, and Olivares, Jose A. Combined electrophoresis-electrospray interface and method. United States: N. p., 1989.
Web.
Smith, Richard P, Udseth, Harold R, & Olivares, Jose A. Combined electrophoresis-electrospray interface and method. United States.
Smith, Richard P, Udseth, Harold R, and Olivares, Jose A. Sun .
"Combined electrophoresis-electrospray interface and method". United States. https://www.osti.gov/servlets/purl/867199.
@article{osti_867199,
title = {Combined electrophoresis-electrospray interface and method},
author = {Smith, Richard P and Udseth, Harold R and Olivares, Jose A},
abstractNote = {A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}