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Title: Flow cytomeric measurement of DNA and incorporated nucleoside analogs

Abstract

A method is provided for simultaneously measuring total cellular DNA and incorporated nucleoside analog. The method entails altering the cellular DNA of cells grown in the presence of a nucleoside analog so that single stranded and double stranded portions are present. Separate stains are used against the two portions. An immunochemical stain is used against the single stranded portion to provide a measure of incorporated nucleoside analog, and a double strand DNA-specific stain is used against the double stranded portion to simultaneously provide a measure of total cellular DNA. The method permits rapid flow cytometric analysis of cell populations, rapid identification of cycling and noncycling subpopulations, and determination of the efficacy of S phase cytotoxic anticancer agents.

Inventors:
 [1];  [1]
  1. (Livermore, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
866875
Patent Number(s):
4812394
Assignee:
University of California (Berkeley, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
flow; cytomeric; measurement; dna; incorporated; nucleoside; analogs; method; provided; simultaneously; measuring; total; cellular; analog; entails; altering; cells; grown; presence; single; stranded; double; portions; separate; stains; immunochemical; stain; portion; provide; measure; strand; dna-specific; permits; rapid; cytometric; analysis; cell; populations; identification; cycling; noncycling; subpopulations; determination; efficacy; phase; cytotoxic; anticancer; agents; single stranded; simultaneously measuring; method entails; method permits; cellular dna; flow cytometric; double stranded; simultaneously provide; nucleoside analog; cell populations; permits rapid; nucleoside analogs; incorporated nucleoside; single strand; cells grown; cells grow; /435/436/999/

Citation Formats

Dolbeare, Frank A., and Gray, Joe W. Flow cytomeric measurement of DNA and incorporated nucleoside analogs. United States: N. p., 1989. Web.
Dolbeare, Frank A., & Gray, Joe W. Flow cytomeric measurement of DNA and incorporated nucleoside analogs. United States.
Dolbeare, Frank A., and Gray, Joe W. Sun . "Flow cytomeric measurement of DNA and incorporated nucleoside analogs". United States. https://www.osti.gov/servlets/purl/866875.
@article{osti_866875,
title = {Flow cytomeric measurement of DNA and incorporated nucleoside analogs},
author = {Dolbeare, Frank A. and Gray, Joe W.},
abstractNote = {A method is provided for simultaneously measuring total cellular DNA and incorporated nucleoside analog. The method entails altering the cellular DNA of cells grown in the presence of a nucleoside analog so that single stranded and double stranded portions are present. Separate stains are used against the two portions. An immunochemical stain is used against the single stranded portion to provide a measure of incorporated nucleoside analog, and a double strand DNA-specific stain is used against the double stranded portion to simultaneously provide a measure of total cellular DNA. The method permits rapid flow cytometric analysis of cell populations, rapid identification of cycling and noncycling subpopulations, and determination of the efficacy of S phase cytotoxic anticancer agents.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1989},
month = {1}
}

Patent:

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