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Title: Single molecule tracking

Abstract

A detection system is provided for identifying individual particles or molecules having characteristic emission in a flow train of the particles in a flow cell. A position sensitive sensor is located adjacent the flow cell in a position effective to detect the emissions from the particles within the flow cell and to assign spatial and temporal coordinates for the detected emissions. A computer is then enabled to predict spatial and temporal coordinates for the particle in the flow train as a function of a first detected emission. Comparison hardware or software then compares subsequent detected spatial and temporal coordinates with the predicted spatial and temporal coordinates to determine whether subsequently detected emissions originate from a particle in the train of particles. In one embodiment, the particles include fluorescent dyes which are excited to fluoresce a spectrum characteristic of the particular particle. Photones are emitted adjacent at least one microchannel plate sensor to enable spatial and temporal coordinates to be assigned. The effect of comparing detected coordinates with predicted coordinates is to define a moving sample volume which effectively precludes the effects of background emissions.

Inventors:
 [1]
  1. (Los Alamos, NM)
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
OSTI Identifier:
866801
Patent Number(s):
4793705
Assignee:
United States of America as represented by United States (Washington, DC) LANL
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
single; molecule; tracking; detection; provided; identifying; individual; particles; molecules; characteristic; emission; flow; train; cell; position; sensitive; sensor; located; adjacent; effective; detect; emissions; spatial; temporal; coordinates; detected; computer; enabled; predict; particle; function; comparison; hardware; software; compares; subsequent; predicted; determine; subsequently; originate; embodiment; fluorescent; dyes; excited; fluoresce; spectrum; particular; photones; emitted; microchannel; plate; enable; assigned; effect; comparing; define; moving; sample; volume; effectively; precludes; effects; background; channel plate; individual particle; background emissions; position sensitive; flow cell; located adjacent; microchannel plate; fluorescent dyes; fluorescent dye; particular particle; sample volume; individual particles; single molecule; sensitive sensor; /356/250/

Citation Formats

Shera, E. Brooks. Single molecule tracking. United States: N. p., 1988. Web.
Shera, E. Brooks. Single molecule tracking. United States.
Shera, E. Brooks. Fri . "Single molecule tracking". United States. https://www.osti.gov/servlets/purl/866801.
@article{osti_866801,
title = {Single molecule tracking},
author = {Shera, E. Brooks},
abstractNote = {A detection system is provided for identifying individual particles or molecules having characteristic emission in a flow train of the particles in a flow cell. A position sensitive sensor is located adjacent the flow cell in a position effective to detect the emissions from the particles within the flow cell and to assign spatial and temporal coordinates for the detected emissions. A computer is then enabled to predict spatial and temporal coordinates for the particle in the flow train as a function of a first detected emission. Comparison hardware or software then compares subsequent detected spatial and temporal coordinates with the predicted spatial and temporal coordinates to determine whether subsequently detected emissions originate from a particle in the train of particles. In one embodiment, the particles include fluorescent dyes which are excited to fluoresce a spectrum characteristic of the particular particle. Photones are emitted adjacent at least one microchannel plate sensor to enable spatial and temporal coordinates to be assigned. The effect of comparing detected coordinates with predicted coordinates is to define a moving sample volume which effectively precludes the effects of background emissions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {1}
}

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