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Title: Method and apparatus for nondestructive in vivo measurement of photosynthesis

A device for in situ, nondestructive measurement of photosynthesis in live plants and photosynthetic microorganisms is disclosed which comprises a Clark-type oxygen electrode having a substantially transparent cathode comprised of an optical fiber having a metallic grid microetched onto its front face and sides, an anode, a substantially transparent electrolyte film, and a substantially transparent oxygen permeable membrane. The device is designed to be placed in direct contact with a photosynthetic portion of a living plant, and nondestructive, noninvasive measurement of photosynthetic oxygen production from the plant can be taken by passing light through the fiber-optic cathode, transparent electroyte and transparent membrane, and onto the plant so that photosynthesis occurs. The oxygen thus produced by the plant is measured polargraphically by the electrode. The present invention allows for rapid, nondestructive measurements of photosynthesis in living plants in a manner heretofore impossible using prior art methods.
  1. (Oak Ridge, TN)
Issue Date:
OSTI Identifier:
United States of America as represented by United States (Washington, DC) ORNL
Patent Number(s):
US 4789436
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN
Country of Publication:
United States
method; apparatus; nondestructive; vivo; measurement; photosynthesis; device; situ; live; plants; photosynthetic; microorganisms; disclosed; comprises; clark-type; oxygen; electrode; substantially; transparent; cathode; comprised; optical; fiber; metallic; grid; microetched; front; anode; electrolyte; film; permeable; membrane; designed; placed; direct; contact; portion; living; plant; noninvasive; production; passing; light; fiber-optic; electroyte; occurs; produced; measured; polargraphically; allows; rapid; measurements; manner; heretofore; impossible; prior; methods; oxygen permeable; oxygen electrode; substantially transparent; electrolyte film; oxygen production; permeable membrane; direct contact; optical fiber; transparent membrane; noninvasive measurement; nondestructive measurement; cathode comprised; vivo measurement; oxygen product; /205/204/435/