skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Electromicroinjection of particles into living cells

Abstract

Method and apparatus for introducing particles into living cells. Fluorescently-stained human chromosomes are introduced into cultured, mitotic Chinese hamster cells using electromicroinjection. The recipient cells frequently survived the physiological perturbation imposed by a successful chromosome injection. Successfully injected recipient cells maintained viability as evidenced by their ability to be expanded. The technique relies on the surface charge of fluorescently stained chromosomes and their ability to be attracted and repelled to and from the tip of a micropipette. The apparatus includes a micropipette having a tip suitable for piercing the membrane of a target cell and an electrode inserted into the lumen thereof. The target cells and suspended particles are located in an electrically conducted solution, and the lumen of the micropipette is filled with an electrically conducting solution which contacts the electrode located therein. A second electrode is also located in the conducting solution containing the target cells and particles. Voltages applied to the electrode within the micropipette attract the particles to the region of the tip thereof. The particles adhere to the surface of the micropipette with sufficient force that insertion of the micropipette tip and attached particle through the membrane of a target cell will not dislodge themore » particle. By applying a voltage having the opposite polarity of the attraction voltage, the particles are expelled from the micropipette to which is then withdrawn from the cell body.« less

Inventors:
 [1];  [1];  [2]
  1. (Los Alamos, NM)
  2. (Albuquerque, NM)
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
OSTI Identifier:
866774
Patent Number(s):
4784737
Assignee:
United States Department of Energy (Washington, DC) LANL
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
electromicroinjection; particles; living; cells; method; apparatus; introducing; fluorescently-stained; human; chromosomes; introduced; cultured; mitotic; chinese; hamster; recipient; frequently; survived; physiological; perturbation; imposed; successful; chromosome; injection; successfully; injected; maintained; viability; evidenced; ability; expanded; technique; relies; surface; charge; fluorescently; stained; attracted; repelled; tip; micropipette; suitable; piercing; membrane; target; cell; electrode; inserted; lumen; suspended; located; electrically; conducted; solution; filled; conducting; contacts; therein; containing; voltages; applied; attract; region; adhere; sufficient; force; insertion; attached; particle; dislodge; applying; voltage; opposite; polarity; attraction; expelled; withdrawn; voltages applied; surface charge; living cells; opposite polarity; solution containing; electrically conducting; located therein; electrode located; sufficient force; suspended particles; human chromosomes; ched particle; /435/604/

Citation Formats

Ray, F. Andrew, Cram, L. Scott, and Galey, William R. Electromicroinjection of particles into living cells. United States: N. p., 1988. Web.
Ray, F. Andrew, Cram, L. Scott, & Galey, William R. Electromicroinjection of particles into living cells. United States.
Ray, F. Andrew, Cram, L. Scott, and Galey, William R. Fri . "Electromicroinjection of particles into living cells". United States. https://www.osti.gov/servlets/purl/866774.
@article{osti_866774,
title = {Electromicroinjection of particles into living cells},
author = {Ray, F. Andrew and Cram, L. Scott and Galey, William R.},
abstractNote = {Method and apparatus for introducing particles into living cells. Fluorescently-stained human chromosomes are introduced into cultured, mitotic Chinese hamster cells using electromicroinjection. The recipient cells frequently survived the physiological perturbation imposed by a successful chromosome injection. Successfully injected recipient cells maintained viability as evidenced by their ability to be expanded. The technique relies on the surface charge of fluorescently stained chromosomes and their ability to be attracted and repelled to and from the tip of a micropipette. The apparatus includes a micropipette having a tip suitable for piercing the membrane of a target cell and an electrode inserted into the lumen thereof. The target cells and suspended particles are located in an electrically conducted solution, and the lumen of the micropipette is filled with an electrically conducting solution which contacts the electrode located therein. A second electrode is also located in the conducting solution containing the target cells and particles. Voltages applied to the electrode within the micropipette attract the particles to the region of the tip thereof. The particles adhere to the surface of the micropipette with sufficient force that insertion of the micropipette tip and attached particle through the membrane of a target cell will not dislodge the particle. By applying a voltage having the opposite polarity of the attraction voltage, the particles are expelled from the micropipette to which is then withdrawn from the cell body.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {1}
}

Patent:

Save / Share: