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Title: Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

Abstract

A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide or Hoechst 33258 is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as halodeoxy-uridine (HdU), more specifically bromodeoxyuridine (BrdU) is used as a probe for the measurement of HdU or BrdU uptake by the cells as a measure of DNA synthesis.

Inventors:
 [1];  [1]
  1. (Livermore, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
866752
Patent Number(s):
4780406
Assignee:
Regents of University of California (Berkeley, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
flow; cytometric; measurement; total; dna; incorporated; halodeoxyuridine; method; simultaneous; content; level; synthesis; normal; malignant; cells; disclosed; sensitivity; allows; study; cell; cycle; traverse; rates; scale; populations; single; measurements; stain; propidium; iodide; hoechst; 33258; probe; monoclonal; antibody; reactive; precursor; halodeoxy-uridine; hdu; specifically; bromodeoxyuridine; brdu; uptake; measure; monoclonal antibody; total dna; dna synthesis; dna content; flow cytometric; single cell; cell cycle; method allows; propidium iodide; malignant cells; cytometric measurement; cell populations; scale cell; simultaneous flow; incorporated halodeoxyuridine; method allow; /435/436/

Citation Formats

Dolbeare, Frank A., and Gray, Joe W. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine. United States: N. p., 1988. Web.
Dolbeare, Frank A., & Gray, Joe W. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine. United States.
Dolbeare, Frank A., and Gray, Joe W. Fri . "Flow cytometric measurement of total DNA and incorporated halodeoxyuridine". United States. https://www.osti.gov/servlets/purl/866752.
@article{osti_866752,
title = {Flow cytometric measurement of total DNA and incorporated halodeoxyuridine},
author = {Dolbeare, Frank A. and Gray, Joe W.},
abstractNote = {A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide or Hoechst 33258 is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as halodeoxy-uridine (HdU), more specifically bromodeoxyuridine (BrdU) is used as a probe for the measurement of HdU or BrdU uptake by the cells as a measure of DNA synthesis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {1}
}

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