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Title: Size-exclusion chromatography system for macromolecular interaction analysis

Abstract

A low pressure, microcomputer controlled system employing high performance liquid chromatography (HPLC) allows for precise analysis of the interaction of two reversibly associating macromolecules such as proteins. Since a macromolecular complex migrates faster than its components during size-exclusion chromatography, the difference between the elution profile of a mixture of two macromolecules and the summation of the elution profiles of the two components provides a quantifiable indication of the degree of molecular interaction. This delta profile is used to qualitatively reveal the presence or absence of significant interaction or to rank the relative degree of interaction in comparing samples and, in combination with a computer simulation, is further used to quantify the magnitude of the interaction in an arrangement wherein a microcomputer is coupled to analytical instrumentation in a novel manner.

Inventors:
 [1]
  1. Downers Grove, IL
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
866670
Patent Number(s):
4762617
Assignee:
United States of America as represented by United States (Washington, DC)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
size-exclusion; chromatography; macromolecular; interaction; analysis; pressure; microcomputer; controlled; employing; performance; liquid; hplc; allows; precise; reversibly; associating; macromolecules; proteins; complex; migrates; faster; components; difference; elution; profile; mixture; summation; profiles; provides; quantifiable; indication; degree; molecular; delta; qualitatively; reveal; presence; absence; significant; rank; relative; comparing; samples; combination; computer; simulation; quantify; magnitude; arrangement; coupled; analytical; instrumentation; novel; manner; performance liquid; liquid chromatograph; computer controlled; liquid chromatography; computer control; size-exclusion chromatography; analytical instrument; computer simulation; /210/73/422/436/530/

Citation Formats

Stevens, Fred J. Size-exclusion chromatography system for macromolecular interaction analysis. United States: N. p., 1988. Web.
Stevens, Fred J. Size-exclusion chromatography system for macromolecular interaction analysis. United States.
Stevens, Fred J. Fri . "Size-exclusion chromatography system for macromolecular interaction analysis". United States. https://www.osti.gov/servlets/purl/866670.
@article{osti_866670,
title = {Size-exclusion chromatography system for macromolecular interaction analysis},
author = {Stevens, Fred J},
abstractNote = {A low pressure, microcomputer controlled system employing high performance liquid chromatography (HPLC) allows for precise analysis of the interaction of two reversibly associating macromolecules such as proteins. Since a macromolecular complex migrates faster than its components during size-exclusion chromatography, the difference between the elution profile of a mixture of two macromolecules and the summation of the elution profiles of the two components provides a quantifiable indication of the degree of molecular interaction. This delta profile is used to qualitatively reveal the presence or absence of significant interaction or to rank the relative degree of interaction in comparing samples and, in combination with a computer simulation, is further used to quantify the magnitude of the interaction in an arrangement wherein a microcomputer is coupled to analytical instrumentation in a novel manner.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {1}
}

Patent:

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