pLS010 plasmid vector
Abstract
Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.
- Inventors:
-
- Brookhaven, NY
- Upton, NY
- Issue Date:
- Research Org.:
- Associated Universities, Inc., Upton, NY (United States)
- OSTI Identifier:
- 866521
- Patent Number(s):
- 4729954
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- pls010; plasmid; vector; disclosed; recombinant; pls101; consisting; essentially; kb; malm; fragment; ligated; dna; particularly; useful; transforming; gram-positive; bacteria; contains; restriction; sites; suitable; inserting; exogeneous; sequences; method; isolation; penicillin; selection; processes; enrichment; plasmids; bacterial; systems; consisting essentially; particularly useful; dna fragment; restriction site; recombinant plasmids; recombinant plasmid; gram-positive bacteria; plasmid contains; /435/
Citation Formats
Lacks, Sanford A, and Balganesh, Tanjore S. pLS010 plasmid vector. United States: N. p., 1988.
Web.
Lacks, Sanford A, & Balganesh, Tanjore S. pLS010 plasmid vector. United States.
Lacks, Sanford A, and Balganesh, Tanjore S. Fri .
"pLS010 plasmid vector". United States. https://www.osti.gov/servlets/purl/866521.
@article{osti_866521,
title = {pLS010 plasmid vector},
author = {Lacks, Sanford A and Balganesh, Tanjore S},
abstractNote = {Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {1}
}
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