Flow cytometer measurement of binding assays
Abstract
A method of measuring the result of a binding assay that does not require separation of fluorescent smaller particles is disclosed. In a competitive binding assay the smaller fluorescent particles coated with antigen compete with antigen in the sample being analyzed for available binding sites on larger particles. In a sandwich assay, the smaller, fluorescent spheres coated with antibody attach themselves to molecules containing antigen that are attached to larger spheres coated with the same antibody. The separation of unattached, fluorescent smaller particles is made unnecessary by only counting the fluorescent events triggered by the laser of a flow cytometer when the event is caused by a particle with a light scatter measurement within a certain range corresponding to the presence of larger particles.
- Inventors:
-
- Espanola, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 866252
- Patent Number(s):
- 4665020
- Assignee:
- United States Department of Energy (Washington, DC)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- flow; cytometer; measurement; binding; assays; method; measuring; result; assay; require; separation; fluorescent; particles; disclosed; competitive; coated; antigen; compete; sample; analyzed; available; sites; larger; sandwich; spheres; antibody; attach; molecules; containing; attached; unattached; unnecessary; counting; events; triggered; laser; event; caused; particle; light; scatter; range; corresponding; presence; binding site; molecules containing; flow cytometer; larger particles; binding sites; particles coated; fluorescent particles; antibody attach; binding assay; /435/436/
Citation Formats
Saunders, George C. Flow cytometer measurement of binding assays. United States: N. p., 1987.
Web.
Saunders, George C. Flow cytometer measurement of binding assays. United States.
Saunders, George C. Thu .
"Flow cytometer measurement of binding assays". United States. https://www.osti.gov/servlets/purl/866252.
@article{osti_866252,
title = {Flow cytometer measurement of binding assays},
author = {Saunders, George C},
abstractNote = {A method of measuring the result of a binding assay that does not require separation of fluorescent smaller particles is disclosed. In a competitive binding assay the smaller fluorescent particles coated with antigen compete with antigen in the sample being analyzed for available binding sites on larger particles. In a sandwich assay, the smaller, fluorescent spheres coated with antibody attach themselves to molecules containing antigen that are attached to larger spheres coated with the same antibody. The separation of unattached, fluorescent smaller particles is made unnecessary by only counting the fluorescent events triggered by the laser of a flow cytometer when the event is caused by a particle with a light scatter measurement within a certain range corresponding to the presence of larger particles.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}