Automated apparatus for producing gradient gels
Abstract
Apparatus for producing a gradient gel which serves as a standard medium for a two-dimensional analysis of proteins, the gel having a density gradient along its height formed by a variation in gel composition, with the apparatus including first and second pumping means each including a plurality of pumps on a common shaft and driven by a stepping motor capable of providing small incremental changes in pump outputs for the gel ingredients, the motors being controlled, by digital signals from a digital computer, a hollow form or cassette for receiving the gel composition, means for transferring the gel composition including a filler tube extending near the bottom of the cassette, adjustable horizontal and vertical arms for automatically removing and relocating the filler tube in the next cassette, and a digital computer programmed to automatically control the stepping motors, arm movements, and associated sensing operations involving the filling operation.
- Inventors:
-
- Clarendon Hills, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 865874
- Patent Number(s):
- 4594064
- Assignee:
- United States of America as represented by United States ()
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- automated; apparatus; producing; gradient; gels; serves; standard; medium; two-dimensional; analysis; proteins; density; height; formed; variation; composition; including; pumping; means; plurality; pumps; common; shaft; driven; stepping; motor; capable; providing; incremental; changes; pump; outputs; ingredients; motors; controlled; digital; signals; computer; hollow; form; cassette; receiving; transferring; filler; tube; extending; near; bottom; adjustable; horizontal; vertical; arms; automatically; removing; relocating; programmed; control; arm; movements; associated; sensing; operations; involving; filling; operation; stepping motor; digital signals; pumping means; digital signal; apparatus including; digital computer; density gradient; tube extending; composition including; automatically control; automated apparatus; motor capable; common shaft; /425/204/
Citation Formats
Anderson, Norman L. Automated apparatus for producing gradient gels. United States: N. p., 1986.
Web.
Anderson, Norman L. Automated apparatus for producing gradient gels. United States.
Anderson, Norman L. Wed .
"Automated apparatus for producing gradient gels". United States. https://www.osti.gov/servlets/purl/865874.
@article{osti_865874,
title = {Automated apparatus for producing gradient gels},
author = {Anderson, Norman L},
abstractNote = {Apparatus for producing a gradient gel which serves as a standard medium for a two-dimensional analysis of proteins, the gel having a density gradient along its height formed by a variation in gel composition, with the apparatus including first and second pumping means each including a plurality of pumps on a common shaft and driven by a stepping motor capable of providing small incremental changes in pump outputs for the gel ingredients, the motors being controlled, by digital signals from a digital computer, a hollow form or cassette for receiving the gel composition, means for transferring the gel composition including a filler tube extending near the bottom of the cassette, adjustable horizontal and vertical arms for automatically removing and relocating the filler tube in the next cassette, and a digital computer programmed to automatically control the stepping motors, arm movements, and associated sensing operations involving the filling operation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}