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Title: Automated apparatus for producing gradient gels

Abstract

Apparatus for producing a gradient gel which serves as a standard medium for a two-dimensional analysis of proteins, the gel having a density gradient along its height formed by a variation in gel composition, with the apparatus including first and second pumping means each including a plurality of pumps on a common shaft and driven by a stepping motor capable of providing small incremental changes in pump outputs for the gel ingredients, the motors being controlled, by digital signals from a digital computer, a hollow form or cassette for receiving the gel composition, means for transferring the gel composition including a filler tube extending near the bottom of the cassette, adjustable horizontal and vertical arms for automatically removing and relocating the filler tube in the next cassette, and a digital computer programmed to automatically control the stepping motors, arm movements, and associated sensing operations involving the filling operation.

Inventors:
 [1]
  1. Clarendon Hills, IL
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL
OSTI Identifier:
865874
Patent Number(s):
4594064
Assignee:
United States of America as represented by United States ()
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
automated; apparatus; producing; gradient; gels; serves; standard; medium; two-dimensional; analysis; proteins; density; height; formed; variation; composition; including; pumping; means; plurality; pumps; common; shaft; driven; stepping; motor; capable; providing; incremental; changes; pump; outputs; ingredients; motors; controlled; digital; signals; computer; hollow; form; cassette; receiving; transferring; filler; tube; extending; near; bottom; adjustable; horizontal; vertical; arms; automatically; removing; relocating; programmed; control; arm; movements; associated; sensing; operations; involving; filling; operation; stepping motor; digital signals; pumping means; digital signal; apparatus including; digital computer; density gradient; tube extending; composition including; automatically control; automated apparatus; motor capable; common shaft; /425/204/

Citation Formats

Anderson, Norman L. Automated apparatus for producing gradient gels. United States: N. p., 1986. Web.
Anderson, Norman L. Automated apparatus for producing gradient gels. United States.
Anderson, Norman L. Wed . "Automated apparatus for producing gradient gels". United States. https://www.osti.gov/servlets/purl/865874.
@article{osti_865874,
title = {Automated apparatus for producing gradient gels},
author = {Anderson, Norman L},
abstractNote = {Apparatus for producing a gradient gel which serves as a standard medium for a two-dimensional analysis of proteins, the gel having a density gradient along its height formed by a variation in gel composition, with the apparatus including first and second pumping means each including a plurality of pumps on a common shaft and driven by a stepping motor capable of providing small incremental changes in pump outputs for the gel ingredients, the motors being controlled, by digital signals from a digital computer, a hollow form or cassette for receiving the gel composition, means for transferring the gel composition including a filler tube extending near the bottom of the cassette, adjustable horizontal and vertical arms for automatically removing and relocating the filler tube in the next cassette, and a digital computer programmed to automatically control the stepping motors, arm movements, and associated sensing operations involving the filling operation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1986},
month = {1}
}

Patent:

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