Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

Title: Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

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Abstract

A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.

Inventors:

Dolbeare, Frank A ^[1]; Gray, Joe W ^[1]

Livermore, CA

Issue Date:: 1986-01-01

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

OSTI Identifier:: 865829

Patent Number(s):: 4585736

Assignee:: United States of America as represented by United States (Washington, DC)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/5308 - {for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
Y10T436/143333 - Saccharide [e.g., DNA, etc.]

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/68 - involving nucleic acids

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DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: flow; cytometric; measurement; total; dna; incorporated; halodeoxyuridine; method; simultaneous; content; level; synthesis; normal; malignant; cells; disclosed; sensitivity; allows; study; cell; cycle; traverse; rates; scale; populations; single; measurements; stain; propidium; iodide; probe; monoclonal; antibody; reactive; precursor; bromodeoxyuridine; brdu; uptake; measure; monoclonal antibody; total dna; dna synthesis; dna content; flow cytometric; single cell; cell cycle; method allows; propidium iodide; malignant cells; cytometric measurement; cell populations; scale cell; simultaneous flow; incorporated halodeoxyuridine; method allow; /435/436/

Citation Formats


                    Dolbeare, Frank A, and Gray, Joe W. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine.  United States: N. p., 1986. 
        Web.

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                    Dolbeare, Frank A, & Gray, Joe W. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine.  United States.

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                    Dolbeare, Frank A, and Gray, Joe W. Wed .  
        "Flow cytometric measurement of total DNA and incorporated halodeoxyuridine".  United States.  https://www.osti.gov/servlets/purl/865829.

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@article{osti_865829,

  title        = {Flow cytometric measurement of total DNA and incorporated halodeoxyuridine},

  author       = {Dolbeare, Frank A and Gray, Joe W},

  abstractNote = {A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1986},

  month        = {1}

}

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