Method of saccharifying cellulose

Title: Method of saccharifying cellulose

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Abstract

A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.

Inventors:

Johnson, Eric A ^[1]; Demain, Arnold L ^[2]; Madia, Ashwin ^[3]

Brookline, MA
Wellesley, MA
Decatur, IL

Issue Date:: 1985-09-10

Research Org.:: Midwest Research Institute, Kansas City, MO (United States)

OSTI Identifier:: 865590

Patent Number(s):: 4540664

Application Number:: 06/494,482

Assignee:: United States of America as represented by United States (Washington, DC)

Patent Classifications (CPCs):: Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/842 - Clostridium

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/14 - produced by the action of a carbohydrase {

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DOE Contract Number:: EG-77-C-02-4198; XR-9-8109-1

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: method; saccharifying; cellulose; incubation; cellulase; clostridium; thermocellum; broth; containing; efficacious; amount; reducing; agent; parameters; advantageously; controlled; stimulate; saccharification; concentration; alkaline; earth; salts; ph; temperature; duration; native; crystalline; found; cotton; completely; saccharified; crystalline cellulose; alkaline earth; reducing agent; earth salts; saccharifying cellulose; clostridium thermocellum; /435/

Citation Formats


                    Johnson, Eric A, Demain, Arnold L, and Madia, Ashwin. Method of saccharifying cellulose.  United States: N. p., 1985. 
        Web.

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                    Johnson, Eric A, Demain, Arnold L, & Madia, Ashwin. Method of saccharifying cellulose.  United States.

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                    Johnson, Eric A, Demain, Arnold L, and Madia, Ashwin. Tue .  
        "Method of saccharifying cellulose".  United States.  https://www.osti.gov/servlets/purl/865590.

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@article{osti_865590,

  title        = {Method of saccharifying cellulose},

  author       = {Johnson, Eric A and Demain, Arnold L and Madia, Ashwin},

  abstractNote = {A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1985},

  month        = {9}

}

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