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Title: Micro-polarimeter for high performance liquid chromatography

Abstract

A micro-polarimeter interfaced with a system for high performance liquid chromatography, for quantitatively analyzing micro and trace amounts of optically active organic molecules, particularly carbohydrates. A flow cell with a narrow bore is connected to a high performance liquid chromatography system. Thin, low birefringence cell windows cover opposite ends of the bore. A focused and polarized laser beam is directed along the longitudinal axis of the bore as an eluent containing the organic molecules is pumped through the cell. The beam is modulated by air gap Faraday rotators for phase sensitive detection to enhance the signal to noise ratio. An analyzer records the beams's direction of polarization after it passes through the cell. Calibration of the liquid chromatography system allows determination of the quantity of organic molecules present from a determination of the degree to which the polarized beam is rotated when it passes through the eluent.

Inventors:
 [1];  [2];  [3];  [4]
  1. (Ames, IA)
  2. (Wilmington, DE)
  3. (Morgantown, WV)
  4. (Skokie, IL)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
865329
Patent Number(s):
4498774
Assignee:
Iowa State University Research Foundation, Inc. (Ames, IA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
micro-polarimeter; performance; liquid; chromatography; interfaced; quantitatively; analyzing; micro; trace; amounts; optically; active; organic; molecules; particularly; carbohydrates; flow; cell; narrow; bore; connected; birefringence; windows; cover; opposite; focused; polarized; laser; beam; directed; longitudinal; axis; eluent; containing; pumped; modulated; air; gap; faraday; rotators; phase; sensitive; detection; enhance; signal; noise; ratio; analyzer; records; beams; direction; polarization; passes; calibration; allows; determination; quantity; degree; rotated; organic molecules; performance liquid; liquid chromatograph; sensitive detection; narrow bore; air gap; laser beam; longitudinal axis; flow cell; liquid chromatography; optically active; polarized beam; polarized laser; noise ratio; quantitatively analyzing; trace amounts; trace amount; phase sensitive; organic molecule; analyzing micro; active organic; /356/422/436/

Citation Formats

Yeung, Edward E., Steenhoek, Larry E., Woodruff, Steven D., and Kuo, Jeng-Chung. Micro-polarimeter for high performance liquid chromatography. United States: N. p., 1985. Web.
Yeung, Edward E., Steenhoek, Larry E., Woodruff, Steven D., & Kuo, Jeng-Chung. Micro-polarimeter for high performance liquid chromatography. United States.
Yeung, Edward E., Steenhoek, Larry E., Woodruff, Steven D., and Kuo, Jeng-Chung. Tue . "Micro-polarimeter for high performance liquid chromatography". United States. https://www.osti.gov/servlets/purl/865329.
@article{osti_865329,
title = {Micro-polarimeter for high performance liquid chromatography},
author = {Yeung, Edward E. and Steenhoek, Larry E. and Woodruff, Steven D. and Kuo, Jeng-Chung},
abstractNote = {A micro-polarimeter interfaced with a system for high performance liquid chromatography, for quantitatively analyzing micro and trace amounts of optically active organic molecules, particularly carbohydrates. A flow cell with a narrow bore is connected to a high performance liquid chromatography system. Thin, low birefringence cell windows cover opposite ends of the bore. A focused and polarized laser beam is directed along the longitudinal axis of the bore as an eluent containing the organic molecules is pumped through the cell. The beam is modulated by air gap Faraday rotators for phase sensitive detection to enhance the signal to noise ratio. An analyzer records the beams's direction of polarization after it passes through the cell. Calibration of the liquid chromatography system allows determination of the quantity of organic molecules present from a determination of the degree to which the polarized beam is rotated when it passes through the eluent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1985},
month = {1}
}

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