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Title: Enhanced growth medium and method for culturing human mammary epithelial cells

Abstract

Methods are disclosed for isolating and culturing human mammary epithelial cells of both normal and malignant origin. Tissue samples are digested with a mixture including the enzymes collagenase and hyaluronidase to produce clumps of cells substantially free from stroma and other undesired cellular material. Growing the clumps of cells in mass culture in an enriched medium containing particular growth factors allows for active cell proliferation and subculture. Clonal culture having plating efficiencies of up to 40% or greater may be obtained using individual cells derived from the mass culture by plating the cells on appropriate substrates in the enriched media. The clonal growth of cells so obtained is suitable for a quantitative assessment of the cytotoxicity of particular treatment. An exemplary assay for assessing the cytotoxicity of the drug adriamycin is presented.

Inventors:
 [1];  [2];  [3]
  1. (7290 Sayre Dr., Oakland, CA 94611)
  2. (5693 Cabot Dr., Oakland, CA 94611)
  3. (82 Evergreen Dr., Orinda, CA 94563)
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA
OSTI Identifier:
864822
Patent Number(s):
4423145
Assignee:
Stampfer, Martha R. (7290 Sayre Dr., Oakland, CA 94611);Smith, Helene S. (5693 Cabot Dr., Oakland, CA 94611);Hackett, Adeline J. (82 Evergreen Dr., Orinda, CA 94563) LBNL
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
enhanced; growth; medium; method; culturing; human; mammary; epithelial; cells; methods; disclosed; isolating; normal; malignant; origin; tissue; samples; digested; mixture; including; enzymes; collagenase; hyaluronidase; produce; clumps; substantially; free; stroma; undesired; cellular; material; growing; mass; culture; enriched; containing; particular; factors; allows; active; cell; proliferation; subculture; clonal; plating; efficiencies; 40; obtained; individual; derived; appropriate; substrates; media; suitable; quantitative; assessment; cytotoxicity; treatment; exemplary; assay; assessing; drug; adriamycin; cellular material; tissue sample; individual cells; substantially free; individual cell; medium containing; mixture including; tissue samples; growth medium; epithelial cells; human mammary; mammary epithelial; active cell; culturing human; /435/

Citation Formats

Stampfer, Martha R., Smith, Helene S., and Hackett, Adeline J. Enhanced growth medium and method for culturing human mammary epithelial cells. United States: N. p., 1983. Web.
Stampfer, Martha R., Smith, Helene S., & Hackett, Adeline J. Enhanced growth medium and method for culturing human mammary epithelial cells. United States.
Stampfer, Martha R., Smith, Helene S., and Hackett, Adeline J. Sat . "Enhanced growth medium and method for culturing human mammary epithelial cells". United States. https://www.osti.gov/servlets/purl/864822.
@article{osti_864822,
title = {Enhanced growth medium and method for culturing human mammary epithelial cells},
author = {Stampfer, Martha R. and Smith, Helene S. and Hackett, Adeline J.},
abstractNote = {Methods are disclosed for isolating and culturing human mammary epithelial cells of both normal and malignant origin. Tissue samples are digested with a mixture including the enzymes collagenase and hyaluronidase to produce clumps of cells substantially free from stroma and other undesired cellular material. Growing the clumps of cells in mass culture in an enriched medium containing particular growth factors allows for active cell proliferation and subculture. Clonal culture having plating efficiencies of up to 40% or greater may be obtained using individual cells derived from the mass culture by plating the cells on appropriate substrates in the enriched media. The clonal growth of cells so obtained is suitable for a quantitative assessment of the cytotoxicity of particular treatment. An exemplary assay for assessing the cytotoxicity of the drug adriamycin is presented.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1983},
month = {1}
}

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