Electronic method for autofluorography of macromolecules on two-D matrices
Abstract
A method for detecting, localizing, and quantifying macromolecules contained in a two-dimensional matrix is provided which employs a television-based position sensitive detection system. A molecule-containing matrix may be produced by conventional means to produce spots of light at the molecule locations which are detected by the television system. The matrix, such as a gel matrix, is exposed to an electronic camera system including an image-intensifier and secondary electron conduction camera capable of light integrating times of many minutes. A light image stored in the form of a charge image on the camera tube target is scanned by conventional television techniques, digitized, and stored in a digital memory. Intensity of any point on the image may be determined from the number at the memory address of the point. The entire image may be displayed on a television monitor for inspection and photographing or individual spots may be analyzed through selected readout of the memory locations. Compared to conventional film exposure methods, the exposure time may be reduced 100-1000 times.
- Inventors:
-
- Oak Ridge, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 864592
- Patent Number(s):
- 4389670
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G01 - MEASURING G01T - MEASUREMENT OF NUCLEAR OR X-RADIATION
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- electronic; method; autofluorography; macromolecules; two-d; matrices; detecting; localizing; quantifying; contained; two-dimensional; matrix; provided; employs; television-based; position; sensitive; detection; molecule-containing; produced; conventional; means; produce; spots; light; molecule; locations; detected; television; exposed; camera; including; image-intensifier; secondary; electron; conduction; capable; integrating; times; minutes; image; stored; form; charge; tube; target; scanned; techniques; digitized; digital; memory; intensity; determined; address; entire; displayed; monitor; inspection; photographing; individual; analyzed; selected; readout; compared; film; exposure; methods; time; reduced; 100-1000; camera tube; sensitive detection; position sensitive; exposure time; secondary electron; camera capable; dimensional matrix; television monitor; light integrating; conventional means; electronic method; electron conduction; memory location; light image; /348/250/376/
Citation Formats
Davidson, Jackson B, and Case, Arthur L. Electronic method for autofluorography of macromolecules on two-D matrices. United States: N. p., 1983.
Web.
Davidson, Jackson B, & Case, Arthur L. Electronic method for autofluorography of macromolecules on two-D matrices. United States.
Davidson, Jackson B, and Case, Arthur L. Sat .
"Electronic method for autofluorography of macromolecules on two-D matrices". United States. https://www.osti.gov/servlets/purl/864592.
@article{osti_864592,
title = {Electronic method for autofluorography of macromolecules on two-D matrices},
author = {Davidson, Jackson B and Case, Arthur L},
abstractNote = {A method for detecting, localizing, and quantifying macromolecules contained in a two-dimensional matrix is provided which employs a television-based position sensitive detection system. A molecule-containing matrix may be produced by conventional means to produce spots of light at the molecule locations which are detected by the television system. The matrix, such as a gel matrix, is exposed to an electronic camera system including an image-intensifier and secondary electron conduction camera capable of light integrating times of many minutes. A light image stored in the form of a charge image on the camera tube target is scanned by conventional television techniques, digitized, and stored in a digital memory. Intensity of any point on the image may be determined from the number at the memory address of the point. The entire image may be displayed on a television monitor for inspection and photographing or individual spots may be analyzed through selected readout of the memory locations. Compared to conventional film exposure methods, the exposure time may be reduced 100-1000 times.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1983},
month = {1}
}