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Title: Fluidic assembly for an ultra-high-speed chromosome flow sorter

Abstract

A fluidic assembly for an ultra-high-speed chromosome flow sorter using a fluid drive system, a nozzle with an orifice having a small ratio of length to diameter, and mechanism for vibrating the nozzle along its axis at high frequencies. The orifice is provided with a sharp edge at its inlet, and a conical section at its outlet for a transition from a short cylindrical aperture of small length to diameter ratio to free space. Sample and sheath fluids in separate low pressure reservoirs are transferred into separate high pressure buffer reservoirs through a valve arrangement which first permit the fluids to be loaded into the buffer reservoirs under low pressure. Once loaded, the buffer reservoirs are subjected to high pressure and valves are operated to permit the buffer reservoirs to be emptied through the nozzle under high pressure. A sensor and decision logic is positioned at the exit of the nozzle, and a charging pulse is applied to the jet when a particle reaches a position further downstream where the droplets are formed. In order to adjust the timing of charge pulses, the distance between the sensing station at the outlet of the nozzle and the droplet breakoff point ismore » determined by stroboscopic illumination of the droplet breakoff region using a laser and a revolving lucite cylinder, and a beam on/off modulator. The breakoff point in the region thus illuminated may then be viewed, using a television monitor.« less

Inventors:
 [1];  [1];  [1]
  1. (Livermore, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
864421
Patent Number(s):
4361400
Assignee:
United States of America as represented by United States (Washington, DC) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
fluidic; assembly; ultra-high-speed; chromosome; flow; sorter; fluid; drive; nozzle; orifice; ratio; length; diameter; mechanism; vibrating; axis; frequencies; provided; sharp; edge; inlet; conical; section; outlet; transition; cylindrical; aperture; free; space; sample; sheath; fluids; separate; pressure; reservoirs; transferred; buffer; valve; arrangement; permit; loaded; subjected; valves; operated; emptied; sensor; decision; logic; positioned; exit; charging; pulse; applied; jet; particle; reaches; position; downstream; droplets; formed; adjust; timing; charge; pulses; distance; sensing; station; droplet; breakoff; determined; stroboscopic; illumination; region; laser; revolving; lucite; cylinder; beam; modulator; illuminated; viewed; television; monitor; charge pulses; sheath fluid; free space; diameter ratio; valve arrangement; television monitor; sharp edge; chromosome flow; ultra-high-speed chromosome; fluidic assembly; flow sorter; fluid drive; decision logic; /256/209/239/

Citation Formats

Gray, Joe W., Alger, Terry W., and Lord, David E. Fluidic assembly for an ultra-high-speed chromosome flow sorter. United States: N. p., 1982. Web.
Gray, Joe W., Alger, Terry W., & Lord, David E. Fluidic assembly for an ultra-high-speed chromosome flow sorter. United States.
Gray, Joe W., Alger, Terry W., and Lord, David E. Fri . "Fluidic assembly for an ultra-high-speed chromosome flow sorter". United States. https://www.osti.gov/servlets/purl/864421.
@article{osti_864421,
title = {Fluidic assembly for an ultra-high-speed chromosome flow sorter},
author = {Gray, Joe W. and Alger, Terry W. and Lord, David E.},
abstractNote = {A fluidic assembly for an ultra-high-speed chromosome flow sorter using a fluid drive system, a nozzle with an orifice having a small ratio of length to diameter, and mechanism for vibrating the nozzle along its axis at high frequencies. The orifice is provided with a sharp edge at its inlet, and a conical section at its outlet for a transition from a short cylindrical aperture of small length to diameter ratio to free space. Sample and sheath fluids in separate low pressure reservoirs are transferred into separate high pressure buffer reservoirs through a valve arrangement which first permit the fluids to be loaded into the buffer reservoirs under low pressure. Once loaded, the buffer reservoirs are subjected to high pressure and valves are operated to permit the buffer reservoirs to be emptied through the nozzle under high pressure. A sensor and decision logic is positioned at the exit of the nozzle, and a charging pulse is applied to the jet when a particle reaches a position further downstream where the droplets are formed. In order to adjust the timing of charge pulses, the distance between the sensing station at the outlet of the nozzle and the droplet breakoff point is determined by stroboscopic illumination of the droplet breakoff region using a laser and a revolving lucite cylinder, and a beam on/off modulator. The breakoff point in the region thus illuminated may then be viewed, using a television monitor.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1982},
month = {1}
}

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