Method and apparatus for electrostatically sorting biological cells
Abstract
An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.
- Inventors:
-
- Berkeley, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 864326
- Patent Number(s):
- 4347935
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; apparatus; electrostatically; sorting; biological; cells; improved; conventional; cell; sorter; generating; fluid; jet; containing; sorted; measuring; distance; centers; adjacent; droplets; zone; defined; separates; descrete; setting; center; droplet; separation; position; optically; sensed; specific; characteristics; integral; multiple; center-to-center; disabling; charger; electrically; charging; detected; optical; sensor; neck; formation; predetermined; fluid jet; droplet formation; improved method; predetermined distance; biological cells; separation zone; optical sensor; sorting biological; integral multiple; biological cell; jet containing; conventional cell; /209/356/377/
Citation Formats
Merrill, John T. Method and apparatus for electrostatically sorting biological cells. United States: N. p., 1982.
Web.
Merrill, John T. Method and apparatus for electrostatically sorting biological cells. United States.
Merrill, John T. Fri .
"Method and apparatus for electrostatically sorting biological cells". United States. https://www.osti.gov/servlets/purl/864326.
@article{osti_864326,
title = {Method and apparatus for electrostatically sorting biological cells},
author = {Merrill, John T},
abstractNote = {An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}