Fluorometric method of quantitative cell mutagenesis
Abstract
A method for assaying a cell culture for mutagenesis is described. A cell culture is stained first with a histochemical stain, and then a fluorescent stain. Normal cells in the culture are stained by both the histochemical and fluorescent stains, while abnormal cells are stained only by the fluorescent stain. The two stains are chosen so that the histochemical stain absorbs the wavelengths that the fluorescent stain emits. After the counterstained culture is subjected to exciting light, the fluorescence from the abnormal cells is detected.
- Inventors:
-
- Livermore, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 864312
- Patent Number(s):
- 4345027
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- fluorometric; method; quantitative; cell; mutagenesis; assaying; culture; described; stained; histochemical; stain; fluorescent; normal; cells; stains; abnormal; chosen; absorbs; wavelengths; emits; counterstained; subjected; exciting; light; fluorescence; detected; normal cells; cell culture; exciting light; /435/427/436/
Citation Formats
Dolbeare, Frank A. Fluorometric method of quantitative cell mutagenesis. United States: N. p., 1982.
Web.
Dolbeare, Frank A. Fluorometric method of quantitative cell mutagenesis. United States.
Dolbeare, Frank A. Fri .
"Fluorometric method of quantitative cell mutagenesis". United States. https://www.osti.gov/servlets/purl/864312.
@article{osti_864312,
title = {Fluorometric method of quantitative cell mutagenesis},
author = {Dolbeare, Frank A},
abstractNote = {A method for assaying a cell culture for mutagenesis is described. A cell culture is stained first with a histochemical stain, and then a fluorescent stain. Normal cells in the culture are stained by both the histochemical and fluorescent stains, while abnormal cells are stained only by the fluorescent stain. The two stains are chosen so that the histochemical stain absorbs the wavelengths that the fluorescent stain emits. After the counterstained culture is subjected to exciting light, the fluorescence from the abnormal cells is detected.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1982},
month = {1}
}
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