Peptide affinity labels for thrombin and other trypsin-like proteases
Abstract
A peptide affinity label of the formula (I): ##STR1## wherein X is a radical capable of acting as a leaving group in a nucleophilic substitution reaction; A is an aromatic amino acid residue; B is H, or a C.sub.1 -C.sub.4 alkyl group, or aryl; Y is selected from the group consisting of hydrogen, aroyl, C.sub.1 -C.sub.6 acyl, and Q--(A)--.sub.n, wherein Q=hydrogen, aroyl, or C.sub.1 -C.sub.6 acyl, n=1-10, A is an amino acid residue selected from the aliphatic, hydroxy-containing, carboxylic acid group, and amide-thereof-containing, aromatic, sulfur-containing and imino-containing amino acids; and wherein J is selected from the group consisting of --CH.sub.2 --, --CH.sub.2 --CH.sub.2 --,--CH.sub.2 --CH.sub.2 --CH.sub.2 --, --CH.dbd.CH-- and --CH(OH)--CH.sub.2. The affinity label is useful for irreversibly inactivating thrombin and trypsin-like enzymes and may be used as a potential anticlotting agent.
- Inventors:
-
- Shoreham, NY
- Yaphank, NY
- Issue Date:
- Research Org.:
- Associated Universities, Inc., Upton, NY (United States)
- OSTI Identifier:
- 864150
- Patent Number(s):
- 4318904
- Application Number:
- 06/143,897
- Assignee:
- Research Corporation (New York, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- peptide; affinity; labels; thrombin; trypsin-like; proteases; label; formula; str1; radical; capable; acting; leaving; nucleophilic; substitution; reaction; aromatic; amino; acid; residue; -c; alkyl; aryl; selected; consisting; hydrogen; aroyl; acyl; q-; 1-10; aliphatic; hydroxy-containing; carboxylic; amide-thereof-containing; sulfur-containing; imino-containing; acids; -ch; dbd; ch-; oh; useful; irreversibly; inactivating; enzymes; potential; anticlotting; agent; amino acid; carboxylic acid; amino acids; nucleophilic substitution; acid residue; peptide affinity; containing amino; substitution reaction; affinity label; /514/530/999/930/
Citation Formats
Shaw, Elliott N, and Kettner, Charles A. Peptide affinity labels for thrombin and other trypsin-like proteases. United States: N. p., 1982.
Web.
Shaw, Elliott N, & Kettner, Charles A. Peptide affinity labels for thrombin and other trypsin-like proteases. United States.
Shaw, Elliott N, and Kettner, Charles A. Tue .
"Peptide affinity labels for thrombin and other trypsin-like proteases". United States. https://www.osti.gov/servlets/purl/864150.
@article{osti_864150,
title = {Peptide affinity labels for thrombin and other trypsin-like proteases},
author = {Shaw, Elliott N and Kettner, Charles A},
abstractNote = {A peptide affinity label of the formula (I): ##STR1## wherein X is a radical capable of acting as a leaving group in a nucleophilic substitution reaction; A is an aromatic amino acid residue; B is H, or a C.sub.1 -C.sub.4 alkyl group, or aryl; Y is selected from the group consisting of hydrogen, aroyl, C.sub.1 -C.sub.6 acyl, and Q--(A)--.sub.n, wherein Q=hydrogen, aroyl, or C.sub.1 -C.sub.6 acyl, n=1-10, A is an amino acid residue selected from the aliphatic, hydroxy-containing, carboxylic acid group, and amide-thereof-containing, aromatic, sulfur-containing and imino-containing amino acids; and wherein J is selected from the group consisting of --CH.sub.2 --, --CH.sub.2 --CH.sub.2 --,--CH.sub.2 --CH.sub.2 --CH.sub.2 --, --CH.dbd.CH-- and --CH(OH)--CH.sub.2. The affinity label is useful for irreversibly inactivating thrombin and trypsin-like enzymes and may be used as a potential anticlotting agent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1982},
month = {3}
}