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Title: Assay for mutagenesis in heterozygous diploid human lymphoblasts

Abstract

An assay is disclosed for determining mutagenic damage caused by the administration of a known or suspected mutagen to diploid human lymphoblastoid cell lines. The gene locus employed for this assay is the gene for thymidine kinase, uridine kinase, or cytidine deaminase. Since human lymphoblastoid cells contain two genes for these enzymes, heterozygotes of human lymphoblastoid cells are used in this assay.

Inventors:
 [1];  [2];  [3];  [3];  [4]
  1. (Somerville, MA)
  2. (Brookline, MA)
  3. (Cambridge, MA)
  4. (Arlington, MA)
Issue Date:
OSTI Identifier:
864055
Patent Number(s):
4302535
Assignee:
Massachusetts Institute of Technology (Cambridge, MA) OSTI
DOE Contract Number:  
EE-77-S-02-4267
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
assay; mutagenesis; heterozygous; diploid; human; lymphoblasts; disclosed; determining; mutagenic; damage; caused; administration; suspected; mutagen; lymphoblastoid; cell; lines; locus; employed; thymidine; kinase; uridine; cytidine; deaminase; cells; contain; genes; enzymes; heterozygotes; cell lines; damage caused; cell line; cells contain; diploid human; /435/

Citation Formats

Skopek, Thomas R., Liber, Howard L., Penman, Bruce W., Thilly, William G., and Hoppe, IV, Henry. Assay for mutagenesis in heterozygous diploid human lymphoblasts. United States: N. p., 1981. Web.
Skopek, Thomas R., Liber, Howard L., Penman, Bruce W., Thilly, William G., & Hoppe, IV, Henry. Assay for mutagenesis in heterozygous diploid human lymphoblasts. United States.
Skopek, Thomas R., Liber, Howard L., Penman, Bruce W., Thilly, William G., and Hoppe, IV, Henry. Thu . "Assay for mutagenesis in heterozygous diploid human lymphoblasts". United States. https://www.osti.gov/servlets/purl/864055.
@article{osti_864055,
title = {Assay for mutagenesis in heterozygous diploid human lymphoblasts},
author = {Skopek, Thomas R. and Liber, Howard L. and Penman, Bruce W. and Thilly, William G. and Hoppe, IV, Henry},
abstractNote = {An assay is disclosed for determining mutagenic damage caused by the administration of a known or suspected mutagen to diploid human lymphoblastoid cell lines. The gene locus employed for this assay is the gene for thymidine kinase, uridine kinase, or cytidine deaminase. Since human lymphoblastoid cells contain two genes for these enzymes, heterozygotes of human lymphoblastoid cells are used in this assay.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1981},
month = {1}
}

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