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Title: Substrates and method for determining enzymes

Abstract

A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.

Inventors:
 [1];  [2]
  1. (574 Escondido Cir., Livermore, CA 94550)
  2. (101 Via Lucia, Alamo, CA 94507)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
864026
Patent Number(s):
4294923
Assignee:
Smith, Robert E. (574 Escondido Cir., Livermore, CA 94550);Bissell, Eugene R. (101 Via Lucia, Alamo, CA 94507) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
substrates; method; determining; enzymes; disclosed; presence; enzyme; biological; fluid; steps; contacting; synthetic; chromogenic; substrate; amino; acid; derivative; 7-amino-4-trifluoromethylcoumarin; incubating; substrate-containing; effect; enzymatic; hydrolysis; fluorometrically; free; chromophore; hydrolyzate; amino acid; enzymatic hydrolysis; biological fluid; acid derivative; containing fluid; /435/930/

Citation Formats

Smith, Robert E., and Bissell, Eugene R. Substrates and method for determining enzymes. United States: N. p., 1981. Web.
Smith, Robert E., & Bissell, Eugene R. Substrates and method for determining enzymes. United States.
Smith, Robert E., and Bissell, Eugene R. Thu . "Substrates and method for determining enzymes". United States. https://www.osti.gov/servlets/purl/864026.
@article{osti_864026,
title = {Substrates and method for determining enzymes},
author = {Smith, Robert E. and Bissell, Eugene R.},
abstractNote = {A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1981},
month = {1}
}

Patent:

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