Substrates and method for determining enzymes
Abstract
A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.
- Inventors:
-
- 574 Escondido Cir., Livermore, CA 94550
- 101 Via Lucia, Alamo, CA 94507
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 864026
- Patent Number(s):
- 4294923
- Assignee:
- Smith, Robert E. (574 Escondido Cir., Livermore, CA 94550);Bissell, Eugene R. (101 Via Lucia, Alamo, CA 94507)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- substrates; method; determining; enzymes; disclosed; presence; enzyme; biological; fluid; steps; contacting; synthetic; chromogenic; substrate; amino; acid; derivative; 7-amino-4-trifluoromethylcoumarin; incubating; substrate-containing; effect; enzymatic; hydrolysis; fluorometrically; free; chromophore; hydrolyzate; amino acid; enzymatic hydrolysis; biological fluid; acid derivative; containing fluid; /435/930/
Citation Formats
Smith, Robert E, and Bissell, Eugene R. Substrates and method for determining enzymes. United States: N. p., 1981.
Web.
Smith, Robert E, & Bissell, Eugene R. Substrates and method for determining enzymes. United States.
Smith, Robert E, and Bissell, Eugene R. Thu .
"Substrates and method for determining enzymes". United States. https://www.osti.gov/servlets/purl/864026.
@article{osti_864026,
title = {Substrates and method for determining enzymes},
author = {Smith, Robert E and Bissell, Eugene R},
abstractNote = {A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1981},
month = {Thu Jan 01 00:00:00 EST 1981}
}
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