Apparatus for continuously referenced analysis of reactive components in solution
Abstract
A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, a reaction zone in fluid communication with said conduit means wherein a first chemical reaction occurs between said species and reactants, and a stream separator disposed within the conduit means for separating the sample solution into a sample stream and a reference stream. An enzymatic reactor is disposed in fluid communication with only the sample stream wherein a second reaction takes place between the said reactants, species, and reactor enzymes causing the consumption or production of an indicator species in just the sample stream. Measurement means such as a photometric system are disposed in communication with the sample and reference streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. A peristaltic pump is provided to equalize flow through the apparatus by evacuation. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids.
- Inventors:
-
- Oak Ridge, TN
- Norris, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 863866
- Patent Number(s):
- 4263406
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- apparatus; continuously; referenced; analysis; reactive; components; solution; measuring; concentration; chemical; species; comprises; combination; conduit; means; introducing; sample; reactants; reaction; zone; fluid; communication; occurs; stream; separator; disposed; separating; reference; enzymatic; reactor; takes; enzymes; causing; consumption; production; indicator; measurement; photometric; streams; outputs; compared; provide; blanked; peristaltic; pump; provided; equalize; flow; evacuation; particularly; suitable; isoenzymes; tissues; fluids; reactive chemical; sample stream; reaction takes; solution comprises; sample solution; particularly suitable; reaction zone; chemical species; chemical reaction; fluid communication; conduit means; reaction occurs; chemical specie; peristaltic pump; active components; reactive components; active chemical; active component; reaction occur; continuously referenced; /435/422/
Citation Formats
Bostick, William D, Denton, Mark S, and Dinsmore, Stanley R. Apparatus for continuously referenced analysis of reactive components in solution. United States: N. p., 1981.
Web.
Bostick, William D, Denton, Mark S, & Dinsmore, Stanley R. Apparatus for continuously referenced analysis of reactive components in solution. United States.
Bostick, William D, Denton, Mark S, and Dinsmore, Stanley R. Thu .
"Apparatus for continuously referenced analysis of reactive components in solution". United States. https://www.osti.gov/servlets/purl/863866.
@article{osti_863866,
title = {Apparatus for continuously referenced analysis of reactive components in solution},
author = {Bostick, William D and Denton, Mark S and Dinsmore, Stanley R},
abstractNote = {A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, a reaction zone in fluid communication with said conduit means wherein a first chemical reaction occurs between said species and reactants, and a stream separator disposed within the conduit means for separating the sample solution into a sample stream and a reference stream. An enzymatic reactor is disposed in fluid communication with only the sample stream wherein a second reaction takes place between the said reactants, species, and reactor enzymes causing the consumption or production of an indicator species in just the sample stream. Measurement means such as a photometric system are disposed in communication with the sample and reference streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. A peristaltic pump is provided to equalize flow through the apparatus by evacuation. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1981},
month = {1}
}