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Title: Apparatus for continuously referenced analysis of reactive components in solution

Abstract

A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, a reaction zone in fluid communication with said conduit means wherein a first chemical reaction occurs between said species and reactants, and a stream separator disposed within the conduit means for separating the sample solution into a sample stream and a reference stream. An enzymatic reactor is disposed in fluid communication with only the sample stream wherein a second reaction takes place between the said reactants, species, and reactor enzymes causing the consumption or production of an indicator species in just the sample stream. Measurement means such as a photometric system are disposed in communication with the sample and reference streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. A peristaltic pump is provided to equalize flow through the apparatus by evacuation. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids.

Inventors:
 [1];  [1];  [2]
  1. (Oak Ridge, TN)
  2. (Norris, TN)
Issue Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN
OSTI Identifier:
863866
Patent Number(s):
4263406
Assignee:
United States of America as represented by United States (Washington, DC) ORNL
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
apparatus; continuously; referenced; analysis; reactive; components; solution; measuring; concentration; chemical; species; comprises; combination; conduit; means; introducing; sample; reactants; reaction; zone; fluid; communication; occurs; stream; separator; disposed; separating; reference; enzymatic; reactor; takes; enzymes; causing; consumption; production; indicator; measurement; photometric; streams; outputs; compared; provide; blanked; peristaltic; pump; provided; equalize; flow; evacuation; particularly; suitable; isoenzymes; tissues; fluids; reactive chemical; sample stream; reaction takes; solution comprises; sample solution; particularly suitable; reaction zone; chemical species; chemical reaction; fluid communication; conduit means; reaction occurs; chemical specie; peristaltic pump; active components; reactive components; active chemical; active component; reaction occur; continuously referenced; /435/422/

Citation Formats

Bostick, William D., Denton, Mark S., and Dinsmore, Stanley R. Apparatus for continuously referenced analysis of reactive components in solution. United States: N. p., 1981. Web.
Bostick, William D., Denton, Mark S., & Dinsmore, Stanley R. Apparatus for continuously referenced analysis of reactive components in solution. United States.
Bostick, William D., Denton, Mark S., and Dinsmore, Stanley R. Thu . "Apparatus for continuously referenced analysis of reactive components in solution". United States. https://www.osti.gov/servlets/purl/863866.
@article{osti_863866,
title = {Apparatus for continuously referenced analysis of reactive components in solution},
author = {Bostick, William D. and Denton, Mark S. and Dinsmore, Stanley R.},
abstractNote = {A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, a reaction zone in fluid communication with said conduit means wherein a first chemical reaction occurs between said species and reactants, and a stream separator disposed within the conduit means for separating the sample solution into a sample stream and a reference stream. An enzymatic reactor is disposed in fluid communication with only the sample stream wherein a second reaction takes place between the said reactants, species, and reactor enzymes causing the consumption or production of an indicator species in just the sample stream. Measurement means such as a photometric system are disposed in communication with the sample and reference streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. A peristaltic pump is provided to equalize flow through the apparatus by evacuation. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1981},
month = {1}
}

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