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Title: Method of freezing living cells and tissues with improved subsequent survival

Abstract

This invention relates to an improved method for freezing red blood cells, ther living cells, or tissues with improved subsequent survival, wherein constant-volume freezing is utilized that results in significantly improved survival compared with constant-pressure freezing; optimization is attainable through the use of different vessel geometries, cooling baths and warming baths, and sample concentrations.

Inventors:
 [1];  [1]
  1. (Oak Ridge, TN)
Issue Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN
OSTI Identifier:
863562
Patent Number(s):
4199022
Assignee:
United States of America as represented by Department of Energy (Washington, DC) ORNL
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; freezing; living; cells; tissues; improved; subsequent; survival; relates; blood; constant-volume; utilized; results; significantly; compared; constant-pressure; optimization; attainable; vessel; geometries; cooling; baths; warming; sample; concentrations; significantly improved; living cells; blood cells; improved method; blood cell; subsequent survival; improved subsequent; significantly improve; /435/62/424/

Citation Formats

Senkan, Selim M., and Hirsch, Gerald P. Method of freezing living cells and tissues with improved subsequent survival. United States: N. p., 1980. Web.
Senkan, Selim M., & Hirsch, Gerald P. Method of freezing living cells and tissues with improved subsequent survival. United States.
Senkan, Selim M., and Hirsch, Gerald P. Tue . "Method of freezing living cells and tissues with improved subsequent survival". United States. https://www.osti.gov/servlets/purl/863562.
@article{osti_863562,
title = {Method of freezing living cells and tissues with improved subsequent survival},
author = {Senkan, Selim M. and Hirsch, Gerald P.},
abstractNote = {This invention relates to an improved method for freezing red blood cells, ther living cells, or tissues with improved subsequent survival, wherein constant-volume freezing is utilized that results in significantly improved survival compared with constant-pressure freezing; optimization is attainable through the use of different vessel geometries, cooling baths and warming baths, and sample concentrations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1980},
month = {1}
}

Patent:

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