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Title: Apparatus for electrophoresis separation

Abstract

An apparatus is disclosed for simultaneously performing electrophoresis separations on a plurality of slab gels containing samples of protein, protein subunits or nucleic acids. A reservoir of buffer solution is divided into three compartments by two parallel partitions having vertical slots spaced along their length. A sheet of flexible, electrically insulative material is attached to each partition and is provided with vertical slits aligned with the slots. Slab-gel holders are received within the slots with the flexible material folded outwardly as flaps from the slits to overlay portions of the holder surfaces and thereby act as electrical and liquid seals. An elongated, spaghetti-like gel containing a sample of specimen that was previously separated by isoelectric focusing techniques is vertically positioned along a marginal edge portion of the slab gel. On application of an electrical potential between the two outer chambers of buffer solution, a second dimensional electrophoresis separation in accordance with molecular weight occurs as the specimen molecules migrate across the slab gel.

Inventors:
 [1]
  1. Willowbrook, IL
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
863079
Patent Number(s):
4088561
Assignee:
United States of America as represented by United States (Washington, DC)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
apparatus; electrophoresis; separation; disclosed; simultaneously; performing; separations; plurality; slab; gels; containing; samples; protein; subunits; nucleic; acids; reservoir; buffer; solution; divided; compartments; parallel; partitions; vertical; slots; spaced; length; sheet; flexible; electrically; insulative; material; attached; partition; provided; slits; aligned; slab-gel; holders; received; folded; outwardly; flaps; overlay; portions; holder; surfaces; electrical; liquid; seals; elongated; spaghetti-like; sample; specimen; previously; separated; isoelectric; focusing; techniques; vertically; positioned; marginal; edge; portion; application; potential; outer; chambers; dimensional; accordance; molecular; weight; occurs; molecules; migrate; liquid seal; electrically insulative; electrical potential; nucleic acid; molecular weight; nucleic acids; buffer solution; edge portion; slab-gel holders; electrophoresis separation; containing sample; insulative material; flexible material; vertical slit; vertical slot; electrophoresis separations; vertically position; slab-gel holder; /204/436/

Citation Formats

Anderson, Norman L. Apparatus for electrophoresis separation. United States: N. p., 1978. Web.
Anderson, Norman L. Apparatus for electrophoresis separation. United States.
Anderson, Norman L. Sun . "Apparatus for electrophoresis separation". United States. https://www.osti.gov/servlets/purl/863079.
@article{osti_863079,
title = {Apparatus for electrophoresis separation},
author = {Anderson, Norman L},
abstractNote = {An apparatus is disclosed for simultaneously performing electrophoresis separations on a plurality of slab gels containing samples of protein, protein subunits or nucleic acids. A reservoir of buffer solution is divided into three compartments by two parallel partitions having vertical slots spaced along their length. A sheet of flexible, electrically insulative material is attached to each partition and is provided with vertical slits aligned with the slots. Slab-gel holders are received within the slots with the flexible material folded outwardly as flaps from the slits to overlay portions of the holder surfaces and thereby act as electrical and liquid seals. An elongated, spaghetti-like gel containing a sample of specimen that was previously separated by isoelectric focusing techniques is vertically positioned along a marginal edge portion of the slab gel. On application of an electrical potential between the two outer chambers of buffer solution, a second dimensional electrophoresis separation in accordance with molecular weight occurs as the specimen molecules migrate across the slab gel.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1978},
month = {1}
}

Patent:

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