Conversion of cellulosic materials to sugar
Abstract
A process for the production of sugar, mainly glucose, by the enzymatic degradation of cellulosic materials, particularly cellulosic wastes, which comprises hydrolyzing the cellulosic material in the presence of cellulase enzyme to produce a sugar solution and recovering from the hydrolysis products a major proportion of the cellulase enzyme used in the hydrolysis reaction for re-use. At least a portion of the required makeup cellulase enzyme is produced in a two-stage operation wherein, in the first stage, a portion of the output sugar solution is utilized to grow a cellulase-secreting microorganism, and, in the second stage, cellulase enzyme formation is induced in the microorganism-containing culture medium by the addition of an appropriate inducer, such as a cellulosic material. Cellulase enzyme is precipitated from the culture liquid by the addition of an organic solvent material, such as a low molecular weight alkyl ketone or alcohol, and the cellulase precipitate is then fed to the hydrolysis reaction.
- Inventors:
-
- El Cerrito, CA
- Berkeley, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 862609
- Patent Number(s):
- 3972775
- Assignee:
- United States of America as represented by United States Energy (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- conversion; cellulosic; materials; sugar; process; production; mainly; glucose; enzymatic; degradation; particularly; wastes; comprises; hydrolyzing; material; presence; cellulase; enzyme; produce; solution; recovering; hydrolysis; products; major; proportion; reaction; re-use; portion; required; makeup; produced; two-stage; operation; stage; output; utilized; grow; cellulase-secreting; microorganism; formation; induced; microorganism-containing; culture; medium; addition; appropriate; inducer; precipitated; liquid; organic; solvent; molecular; weight; alkyl; ketone; alcohol; precipitate; fed; cellulase enzyme; cellulosic material; culture medium; cellulosic materials; organic solvent; molecular weight; cellulosic waste; major proportion; weight alkyl; stage operation; /435/
Citation Formats
Wilke, Charles R, and Mitra, Gautam. Conversion of cellulosic materials to sugar. United States: N. p., 1976.
Web.
Wilke, Charles R, & Mitra, Gautam. Conversion of cellulosic materials to sugar. United States.
Wilke, Charles R, and Mitra, Gautam. Tue .
"Conversion of cellulosic materials to sugar". United States. https://www.osti.gov/servlets/purl/862609.
@article{osti_862609,
title = {Conversion of cellulosic materials to sugar},
author = {Wilke, Charles R and Mitra, Gautam},
abstractNote = {A process for the production of sugar, mainly glucose, by the enzymatic degradation of cellulosic materials, particularly cellulosic wastes, which comprises hydrolyzing the cellulosic material in the presence of cellulase enzyme to produce a sugar solution and recovering from the hydrolysis products a major proportion of the cellulase enzyme used in the hydrolysis reaction for re-use. At least a portion of the required makeup cellulase enzyme is produced in a two-stage operation wherein, in the first stage, a portion of the output sugar solution is utilized to grow a cellulase-secreting microorganism, and, in the second stage, cellulase enzyme formation is induced in the microorganism-containing culture medium by the addition of an appropriate inducer, such as a cellulosic material. Cellulase enzyme is precipitated from the culture liquid by the addition of an organic solvent material, such as a low molecular weight alkyl ketone or alcohol, and the cellulase precipitate is then fed to the hydrolysis reaction.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 03 00:00:00 EDT 1976},
month = {Tue Aug 03 00:00:00 EDT 1976}
}