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Title: Conversion of cellulosic materials to sugar

Abstract

A process for the production of sugar, mainly glucose, by the enzymatic degradation of cellulosic materials, particularly cellulosic wastes, which comprises hydrolyzing the cellulosic material in the presence of cellulase enzyme to produce a sugar solution and recovering from the hydrolysis products a major proportion of the cellulase enzyme used in the hydrolysis reaction for re-use. At least a portion of the required makeup cellulase enzyme is produced in a two-stage operation wherein, in the first stage, a portion of the output sugar solution is utilized to grow a cellulase-secreting microorganism, and, in the second stage, cellulase enzyme formation is induced in the microorganism-containing culture medium by the addition of an appropriate inducer, such as a cellulosic material. Cellulase enzyme is precipitated from the culture liquid by the addition of an organic solvent material, such as a low molecular weight alkyl ketone or alcohol, and the cellulase precipitate is then fed to the hydrolysis reaction.

Inventors:
 [1];  [2]
  1. El Cerrito, CA
  2. Berkeley, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
862609
Patent Number(s):
3972775
Assignee:
United States of America as represented by United States Energy (Washington, DC)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
conversion; cellulosic; materials; sugar; process; production; mainly; glucose; enzymatic; degradation; particularly; wastes; comprises; hydrolyzing; material; presence; cellulase; enzyme; produce; solution; recovering; hydrolysis; products; major; proportion; reaction; re-use; portion; required; makeup; produced; two-stage; operation; stage; output; utilized; grow; cellulase-secreting; microorganism; formation; induced; microorganism-containing; culture; medium; addition; appropriate; inducer; precipitated; liquid; organic; solvent; molecular; weight; alkyl; ketone; alcohol; precipitate; fed; cellulase enzyme; cellulosic material; culture medium; cellulosic materials; organic solvent; molecular weight; cellulosic waste; major proportion; weight alkyl; stage operation; /435/

Citation Formats

Wilke, Charles R, and Mitra, Gautam. Conversion of cellulosic materials to sugar. United States: N. p., 1976. Web.
Wilke, Charles R, & Mitra, Gautam. Conversion of cellulosic materials to sugar. United States.
Wilke, Charles R, and Mitra, Gautam. Tue . "Conversion of cellulosic materials to sugar". United States. https://www.osti.gov/servlets/purl/862609.
@article{osti_862609,
title = {Conversion of cellulosic materials to sugar},
author = {Wilke, Charles R and Mitra, Gautam},
abstractNote = {A process for the production of sugar, mainly glucose, by the enzymatic degradation of cellulosic materials, particularly cellulosic wastes, which comprises hydrolyzing the cellulosic material in the presence of cellulase enzyme to produce a sugar solution and recovering from the hydrolysis products a major proportion of the cellulase enzyme used in the hydrolysis reaction for re-use. At least a portion of the required makeup cellulase enzyme is produced in a two-stage operation wherein, in the first stage, a portion of the output sugar solution is utilized to grow a cellulase-secreting microorganism, and, in the second stage, cellulase enzyme formation is induced in the microorganism-containing culture medium by the addition of an appropriate inducer, such as a cellulosic material. Cellulase enzyme is precipitated from the culture liquid by the addition of an organic solvent material, such as a low molecular weight alkyl ketone or alcohol, and the cellulase precipitate is then fed to the hydrolysis reaction.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1976},
month = {8}
}

Patent:

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