Substrates and method for determining enzymes
Abstract
A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate. No Drawings
- Inventors:
- Issue Date:
- OSTI Identifier:
- 7254143
- Patent Number(s):
- 4294923
- Application Number:
- PPN: US 6-032444
- Assignee:
- PTO; EDB-94-125890
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 23 Apr 1979
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ENZYMES; MEASURING METHODS; BIOCHEMISTRY; BODY FLUIDS; ENZYMATIC HYDROLYSIS; BIOLOGICAL MATERIALS; CHEMICAL REACTIONS; CHEMISTRY; DECOMPOSITION; HYDROLYSIS; LYSIS; MATERIALS; ORGANIC COMPOUNDS; PROTEINS; SOLVOLYSIS; 550200* - Biochemistry
Citation Formats
Smith, R E, and Bissell, E R. Substrates and method for determining enzymes. United States: N. p., 1981.
Web.
Smith, R E, & Bissell, E R. Substrates and method for determining enzymes. United States.
Smith, R E, and Bissell, E R. Tue .
"Substrates and method for determining enzymes". United States.
@article{osti_7254143,
title = {Substrates and method for determining enzymes},
author = {Smith, R E and Bissell, E R},
abstractNote = {A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate. No Drawings},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 13 00:00:00 EDT 1981},
month = {Tue Oct 13 00:00:00 EDT 1981}
}
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