Apparatus for eliminating background interference in fluorescence measurements
Abstract
The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle. 8 figs.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 7242979
- Patent Number(s):
- 4573796
- Application Number:
- PPN: US 6-568768
- Assignee:
- Dept. of Energy, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 6 Jan 1984
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BIOLOGICAL MATERIALS; FLUORESCENCE; CELL FLOW SYSTEMS; INTERFERENCE; MEASURING INSTRUMENTS; DESIGN; LUMINESCENCE; MATERIALS; 550200* - Biochemistry
Citation Formats
Martin, J C, and Jett, J H. Apparatus for eliminating background interference in fluorescence measurements. United States: N. p., 1986.
Web.
Martin, J C, & Jett, J H. Apparatus for eliminating background interference in fluorescence measurements. United States.
Martin, J C, and Jett, J H. Tue .
"Apparatus for eliminating background interference in fluorescence measurements". United States.
@article{osti_7242979,
title = {Apparatus for eliminating background interference in fluorescence measurements},
author = {Martin, J C and Jett, J H},
abstractNote = {The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle. 8 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 04 00:00:00 EST 1986},
month = {Tue Mar 04 00:00:00 EST 1986}
}
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