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Title: Apparatus for eliminating background interference in fluorescence measurements

Abstract

The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle. 8 figs.

Inventors:
;
Issue Date:
OSTI Identifier:
7242979
Patent Number(s):
4573796
Application Number:
PPN: US 6-568768
Assignee:
Dept. of Energy, Washington, DC (United States)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Resource Relation:
Patent File Date: 6 Jan 1984
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BIOLOGICAL MATERIALS; FLUORESCENCE; CELL FLOW SYSTEMS; INTERFERENCE; MEASURING INSTRUMENTS; DESIGN; LUMINESCENCE; MATERIALS; 550200* - Biochemistry

Citation Formats

Martin, J C, and Jett, J H. Apparatus for eliminating background interference in fluorescence measurements. United States: N. p., 1986. Web.
Martin, J C, & Jett, J H. Apparatus for eliminating background interference in fluorescence measurements. United States.
Martin, J C, and Jett, J H. Tue . "Apparatus for eliminating background interference in fluorescence measurements". United States.
@article{osti_7242979,
title = {Apparatus for eliminating background interference in fluorescence measurements},
author = {Martin, J C and Jett, J H},
abstractNote = {The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle. 8 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1986},
month = {3}
}