Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae

Lacks, S A

Title: Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae

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Abstract

Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252. 9 figs.

Inventors:: Lacks, S A

Issue Date:: 1990-10-02

OSTI Identifier:: 7162961

Patent Number(s):: 4960707

Application Number:: PPN: US 7-085944

Assignee:: Associated Universities, Inc., Washington, DC (United States)

DOE Contract Number:: AC02-76CH00016

Resource Type:: Patent

Resource Relation:: Patent File Date: 17 Aug 1987

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; STREPTOCOCCUS; GENETIC ENGINEERING; DNA-CLONING; ENDONUCLEASES; RECOMBINANT DNA; BACTERIA; BIOTECHNOLOGY; CLONING; DNA; DNA HYBRIDIZATION; DNA-ASE; ENZYMES; ESTERASES; HYBRIDIZATION; HYDROLASES; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PROTEINS; 550200* - Biochemistry

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                    Lacks, S A. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae.  United States: N. p., 1990. 
        Web.

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                    Lacks, S A. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae.  United States.

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                    Lacks, S A. Tue .  
        "Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae".  United States.

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@article{osti_7162961,

  title        = {Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae},

  author       = {Lacks, S A},

  abstractNote = {Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252. 9 figs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1990},

  month        = {10}

}

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Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae

Patent Lacks, Sanford A [Brookhaven, NY]

Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252.
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Although a number of bacterial species are naturally transformable, that is, their cells are able to take up external DNA in substantial amounts and integrate it into the chromosome without artificial manipulation of the cell surface, Streptococcus pneumoniae, the first species in which this phenomenon was detected, remains a prototype of such transformation. Cells of S. pneumonias also contain potent restriction endonucleases able to severely restrict DNA introduced during viral infection. Our current understanding of the genetic basis of the complementary DpnI and DpnII restriction systems and of the biochemistry of their component enzymes are briefly reviewed. The manner inmore » « less
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Transformation and cloning of the DpnI and DpnII endonuclease genes has clarified the genetic basis of the two restriction systems. Molecular cloning was carried out in the Gram-positive S. pneumoniae host/vector system. Cloned chromosomal fragments from both DpnI- and DpnII-producing strains were subjected to nucleotide sequence determination and were used as probes for DNA hybridization analysis. It was shown that the restriction enzyme phenotype of S. pneumoniae depended on an intercellular genetic cassette mechanism. In this review some aspects of the evolution of restriction systems in S. pneumoniae and other bacterial will be discussed. 42 refs., 7 figs., 1 tab.
Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae

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