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Title: Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae

Abstract

Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252. 9 figs.

Inventors:
Issue Date:
OSTI Identifier:
7162961
Patent Number(s):
4960707 A
Application Number:
PPN: US 7-085944
Assignee:
Associated Universities, Inc., Washington, DC (United States) PTO; EDB-94-118898
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Resource Relation:
Patent File Date: 17 Aug 1987
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; STREPTOCOCCUS; GENETIC ENGINEERING; DNA-CLONING; ENDONUCLEASES; RECOMBINANT DNA; BACTERIA; BIOTECHNOLOGY; CLONING; DNA; DNA HYBRIDIZATION; DNA-ASE; ENZYMES; ESTERASES; HYBRIDIZATION; HYDROLASES; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PROTEINS; 550200* - Biochemistry

Citation Formats

Lacks, S.A. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae. United States: N. p., 1990. Web.
Lacks, S.A. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae. United States.
Lacks, S.A. Tue . "Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae". United States.
@article{osti_7162961,
title = {Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae},
author = {Lacks, S.A.},
abstractNote = {Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252. 9 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {10}
}