skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Refractive index and absorption detector for liquid chromatography based on Fabry-Perot interferometry

Abstract

A refractive index and absorption detector are disclosed for liquid chromatography. It is based in part on a Fabry-Perot interferometer and is used for the improved detection of refractive index and absorption. It includes a Fabry-Perot interferometer having a normally fixed first partially reflecting mirror and a movable second partially reflecting mirror. A chromatographic flow-cell is positioned between the mirrors along the optical axis of a monochromatic laser beam passing through the interferometer. A means for deriving information about the interference fringes coming out of the interferometer is used with a mini-computer to compute the refractive index of the specimen injected into the flow cell. The minicomputer continuously scans the interferometer for continuous refractive index readings and outputs the continuous results of the scans on a chart recorder. The absorption of the specimen can concurrently be scanned by including a second optical path for an excitation laser which will not interfere with the first laser, but will affect the specimen so that absorption properties can be detected. By first scanning for the refractive index of the specimen, and then immediately adding the excitation laser and subsequently scanning for the refractive index again, the absorption of the specimen can be computedmore » and recorded. 10 figs.« less

Inventors:
;
Issue Date:
OSTI Identifier:
7148107
Patent Number(s):
4455089 A
Application Number:
PPN: US 6-460467
Assignee:
Iowa State Univ. Research Foundation, Inc., Ames, IA (United States) PTO; EDB-94-124743
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Patent
Resource Relation:
Patent File Date: 24 Jan 1983
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; FABRY-PEROT INTERFEROMETER; DESIGN; LIQUID COLUMN CHROMATOGRAPHY; MONITORING; LIQUIDS; ABSORPTION; REFRACTIVE INDEX; LASER RADIATION; CHROMATOGRAPHY; ELECTROMAGNETIC RADIATION; FLUIDS; INTERFEROMETERS; MEASURING INSTRUMENTS; OPTICAL PROPERTIES; PHYSICAL PROPERTIES; RADIATIONS; SEPARATION PROCESSES; SORPTION; 400102* - Chemical & Spectral Procedures; 400105 - Separation Procedures

Citation Formats

Yeung, E.S., and Woodruff, S.D. Refractive index and absorption detector for liquid chromatography based on Fabry-Perot interferometry. United States: N. p., 1984. Web.
Yeung, E.S., & Woodruff, S.D. Refractive index and absorption detector for liquid chromatography based on Fabry-Perot interferometry. United States.
Yeung, E.S., and Woodruff, S.D. Tue . "Refractive index and absorption detector for liquid chromatography based on Fabry-Perot interferometry". United States.
@article{osti_7148107,
title = {Refractive index and absorption detector for liquid chromatography based on Fabry-Perot interferometry},
author = {Yeung, E.S. and Woodruff, S.D.},
abstractNote = {A refractive index and absorption detector are disclosed for liquid chromatography. It is based in part on a Fabry-Perot interferometer and is used for the improved detection of refractive index and absorption. It includes a Fabry-Perot interferometer having a normally fixed first partially reflecting mirror and a movable second partially reflecting mirror. A chromatographic flow-cell is positioned between the mirrors along the optical axis of a monochromatic laser beam passing through the interferometer. A means for deriving information about the interference fringes coming out of the interferometer is used with a mini-computer to compute the refractive index of the specimen injected into the flow cell. The minicomputer continuously scans the interferometer for continuous refractive index readings and outputs the continuous results of the scans on a chart recorder. The absorption of the specimen can concurrently be scanned by including a second optical path for an excitation laser which will not interfere with the first laser, but will affect the specimen so that absorption properties can be detected. By first scanning for the refractive index of the specimen, and then immediately adding the excitation laser and subsequently scanning for the refractive index again, the absorption of the specimen can be computed and recorded. 10 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1984},
month = {6}
}