Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae
Abstract
A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of Streptococcus pneumoniae. Plasmid pSM22, the vector containing the pneumocccal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 figure.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 7026683
- Patent Number(s):
- 5002875
- Application Number:
- PPN: US 7-090623
- Assignee:
- Dept. of Energy, Washington, DC (United States)
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 28 Aug 1987
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; DNA POLYMERASES; GENES; PLASMIDS; BIOLOGICAL FUNCTIONS; DNA-CLONING; CELL CONSTITUENTS; CLONING; DNA HYBRIDIZATION; ENZYMES; HYBRIDIZATION; NUCLEOTIDYLTRANSFERASES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; PROTEINS; TRANSFERASES; 550200* - Biochemistry
Citation Formats
Lacks, S A, Martinez, S, Lopez, P, and Espinosa, M. Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae. United States: N. p., 1991.
Web.
Lacks, S A, Martinez, S, Lopez, P, & Espinosa, M. Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae. United States.
Lacks, S A, Martinez, S, Lopez, P, and Espinosa, M. Tue .
"Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae". United States.
@article{osti_7026683,
title = {Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae},
author = {Lacks, S A and Martinez, S and Lopez, P and Espinosa, M},
abstractNote = {A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of Streptococcus pneumoniae. Plasmid pSM22, the vector containing the pneumocccal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 figure.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 26 00:00:00 EST 1991},
month = {Tue Mar 26 00:00:00 EST 1991}
}
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