Combined electrophoresis-electrospray interface and method
Abstract
A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5--100 kVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., [+-]2--8 kVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit. 21 figs.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 7019966
- Patent Number(s):
- 4885076 A
- Application Number:
- PPN: US 7-178046
- Assignee:
- Battelle Memorial Inst., Richland, WA (United States) PTO; EDB-94-117214
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 5 Apr 1988
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; SOLUTIONS; ATOMIZATION; CHEMICAL ANALYSIS; ELECTROPHORESIS; ELECTRIC POTENTIAL; DISPERSIONS; MIXTURES; 400102* - Chemical & Spectral Procedures
Citation Formats
Smith, R.P., Udseth, H.R., and Olivares, J.A. Combined electrophoresis-electrospray interface and method. United States: N. p., 1989.
Web.
Smith, R.P., Udseth, H.R., & Olivares, J.A. Combined electrophoresis-electrospray interface and method. United States.
Smith, R.P., Udseth, H.R., and Olivares, J.A. Tue .
"Combined electrophoresis-electrospray interface and method". United States.
@article{osti_7019966,
title = {Combined electrophoresis-electrospray interface and method},
author = {Smith, R.P. and Udseth, H.R. and Olivares, J.A.},
abstractNote = {A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5--100 kVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., [+-]2--8 kVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit. 21 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1989},
month = {12}
}