Combined electrophoretic-separation and electrospray method and system
Abstract
A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary zone electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5--100 kVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., [+-]2--8 kVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit. 10 figs.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 7019926
- Patent Number(s):
- 4842701
- Application Number:
- PPN: US 7-034875
- Assignee:
- Battelle Memorial Inst., Richland, WA (United States)
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 6 Apr 1987
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; SOLUTIONS; CHEMICAL ANALYSIS; ELECTROPHORESIS; ELECTROSTATIC SEPARATION; CHEMICAL COMPOSITION; ELECTRIC POTENTIAL; SPRAYS; DISPERSIONS; MIXTURES; SEPARATION PROCESSES; 400105* - Separation Procedures
Citation Formats
Smith, R D, and Olivares, J A. Combined electrophoretic-separation and electrospray method and system. United States: N. p., 1989.
Web.
Smith, R D, & Olivares, J A. Combined electrophoretic-separation and electrospray method and system. United States.
Smith, R D, and Olivares, J A. Tue .
"Combined electrophoretic-separation and electrospray method and system". United States.
@article{osti_7019926,
title = {Combined electrophoretic-separation and electrospray method and system},
author = {Smith, R D and Olivares, J A},
abstractNote = {A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary zone electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5--100 kVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., [+-]2--8 kVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit. 10 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1989},
month = {6}
}