Quantitative luminescence imaging system
Abstract
The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shapedmore »
- Inventors:
- Issue Date:
- OSTI Identifier:
- 7016834
- Patent Number(s):
- 4948975
- Application Number:
- PPN: US 7-241992
- Assignee:
- Air Force, Washington, DC (United States)
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 8 Sep 1988
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; IMAGE PROCESSING; DIGITIZERS; DESIGN; IMAGE INTENSIFIERS; IMMUNOASSAY; REAL TIME SYSTEMS; SAMPLE PREPARATION; BIOASSAY; ELECTRONIC CIRCUITS; PROCESSING; PULSE CIRCUITS; SIGNAL CONDITIONERS; 550200* - Biochemistry; 550300 - Cytology
Citation Formats
Erwin, D N, Kiel, J L, Batishko, C R, and Stahl, K A. Quantitative luminescence imaging system. United States: N. p., 1990.
Web.
Erwin, D N, Kiel, J L, Batishko, C R, & Stahl, K A. Quantitative luminescence imaging system. United States.
Erwin, D N, Kiel, J L, Batishko, C R, and Stahl, K A. Tue .
"Quantitative luminescence imaging system". United States.
@article{osti_7016834,
title = {Quantitative luminescence imaging system},
author = {Erwin, D N and Kiel, J L and Batishko, C R and Stahl, K A},
abstractNote = {The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shaped sample cell, (2) RF irradiation of samples in a microscope for the microscopic imaging and measurement, and (3) RF irradiation of small to human body-sized samples in an anechoic chamber. 22 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {8}
}