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Title: Peptide affinity labels for thrombin and other trypsin-like proteases

Abstract

A peptide affinity label is disclosed of the formula (I): as given in the patent wherein X is a radical capable of acting as a leaving group in a nucleophilic substitution reaction; A is an aromatic amino acid residue; B is H, or a C[sub 1]--C[sub 4] alkyl group, or aryl; Y is selected from the group consisting of hydrogen, aroyl, C[sub 1]--C[sub 6] acyl, and Q--(A)--[sub n], wherein Q = hydrogen, aroyl, or C[sub 1]--C[sub 6] acyl, n = 1--10, A is an amino acid residue selected from the aliphatic, hydroxy-containing, carboxylic acid group, and amide-thereofcontaining, aromatic, sulfur-containing and imino-containing amino acids; and wherein J is selected from the group consisting of --CH[sub 2]--, --CH[sub 2]--CH[sub 2]--, --CH[sub 2]--CH[sub 2]--CH[sub 2]--, --CH[double bond]CH-- and --CH(OH)--CH[sub 2]. The affinity label is useful for irreversibly inactivating thrombin and trypsin-like enzymes and may be used as a potential anticlotting agent. 2 figs.

Inventors:
;
Issue Date:
OSTI Identifier:
7000912
Patent Number(s):
4318904 A
Application Number:
PPN: US 6-143897
Assignee:
Research Corp., New York, NY (United States) PTO; EDB-94-125896
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Resource Relation:
Patent File Date: 25 Apr 1980
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDE HYDROLASES; LABELLING; PEPTIDES; BIOCHEMICAL REACTION KINETICS; THROMBIN; CHEMICAL COMPOSITION; BLOOD COAGULATION FACTORS; COAGULANTS; DRUGS; ENZYMES; HEMATOLOGIC AGENTS; HEMOSTATICS; HYDROLASES; KINETICS; ORGANIC COMPOUNDS; PROTEINS; REACTION KINETICS; SERINE PROTEINASES; 550200* - Biochemistry

Citation Formats

Shaw, E.N., and Kettner, C.A. Peptide affinity labels for thrombin and other trypsin-like proteases. United States: N. p., 1982. Web.
Shaw, E.N., & Kettner, C.A. Peptide affinity labels for thrombin and other trypsin-like proteases. United States.
Shaw, E.N., and Kettner, C.A. Tue . "Peptide affinity labels for thrombin and other trypsin-like proteases". United States.
@article{osti_7000912,
title = {Peptide affinity labels for thrombin and other trypsin-like proteases},
author = {Shaw, E.N. and Kettner, C.A.},
abstractNote = {A peptide affinity label is disclosed of the formula (I): as given in the patent wherein X is a radical capable of acting as a leaving group in a nucleophilic substitution reaction; A is an aromatic amino acid residue; B is H, or a C[sub 1]--C[sub 4] alkyl group, or aryl; Y is selected from the group consisting of hydrogen, aroyl, C[sub 1]--C[sub 6] acyl, and Q--(A)--[sub n], wherein Q = hydrogen, aroyl, or C[sub 1]--C[sub 6] acyl, n = 1--10, A is an amino acid residue selected from the aliphatic, hydroxy-containing, carboxylic acid group, and amide-thereofcontaining, aromatic, sulfur-containing and imino-containing amino acids; and wherein J is selected from the group consisting of --CH[sub 2]--, --CH[sub 2]--CH[sub 2]--, --CH[sub 2]--CH[sub 2]--CH[sub 2]--, --CH[double bond]CH-- and --CH(OH)--CH[sub 2]. The affinity label is useful for irreversibly inactivating thrombin and trypsin-like enzymes and may be used as a potential anticlotting agent. 2 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1982},
month = {3}
}