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Title: Method for rapid base sequencing in DNA and RNA

Abstract

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed. 2 figs.

Inventors:
; ; ; ; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley Lab., CA (USA)
OSTI Identifier:
6864735
Patent Number(s):
-US-A6105375
Assignee:
LBNL; EDB-88-179642
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA SEQUENCING; MODIFICATIONS; DYES; EVALUATION; GENES; LABELLING; GENETICS; INVENTIONS; NUCLEASES; OPERATION; RNA; BIOLOGY; ENZYMES; ESTERASES; HYDROLASES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; STRUCTURAL CHEMICAL ANALYSIS 550200* -- Biochemistry

Citation Formats

Jett, J.H., Keller, R.A., Martin, J.C., Moyzis, R.K., Ratliff, R.L., Shera, E.B., and Stewart, C.C. Method for rapid base sequencing in DNA and RNA. United States: N. p., 1987. Web.
Jett, J.H., Keller, R.A., Martin, J.C., Moyzis, R.K., Ratliff, R.L., Shera, E.B., & Stewart, C.C. Method for rapid base sequencing in DNA and RNA. United States.
Jett, J.H., Keller, R.A., Martin, J.C., Moyzis, R.K., Ratliff, R.L., Shera, E.B., and Stewart, C.C. Wed . "Method for rapid base sequencing in DNA and RNA". United States.
@article{osti_6864735,
title = {Method for rapid base sequencing in DNA and RNA},
author = {Jett, J.H. and Keller, R.A. and Martin, J.C. and Moyzis, R.K. and Ratliff, R.L. and Shera, E.B. and Stewart, C.C.},
abstractNote = {A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed. 2 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1987},
month = {10}
}