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Title: Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae

Abstract

A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of /und Streptococcus/ /und pneumoniae/. Plasmid pSM22, the vector containing the pneumococcal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 fig., 1 tab.

Inventors:
; ; ;
Issue Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
OSTI Identifier:
6805409
Patent Number(s):
6090623
Assignee:
TIC; EDB-88-179643
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENES; DNA-CLONING; BACILLUS SUBTILIS; DNA POLYMERASES; DNA REPLICATION; DNA SEQUENCING; INVENTIONS; PLASMIDS; STREPTOCOCCUS; TRACER TECHNIQUES; BACILLUS; BACTERIA; CELL CONSTITUENTS; CLONING; ENZYMES; ISOTOPE APPLICATIONS; MICROORGANISMS; NUCLEIC ACID REPLICATION; NUCLEOTIDYLTRANSFERASES; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; STRUCTURAL CHEMICAL ANALYSIS; TRANSFERASES; 550200* - Biochemistry

Citation Formats

Lacks, S A, Martinez, S, Lopez, P, and Espinosa, M. Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae. United States: N. p., 1987. Web.
Lacks, S A, Martinez, S, Lopez, P, & Espinosa, M. Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae. United States.
Lacks, S A, Martinez, S, Lopez, P, and Espinosa, M. Fri . "Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae". United States.
@article{osti_6805409,
title = {Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae},
author = {Lacks, S A and Martinez, S and Lopez, P and Espinosa, M},
abstractNote = {A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of /und Streptococcus/ /und pneumoniae/. Plasmid pSM22, the vector containing the pneumococcal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 fig., 1 tab.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1987},
month = {8}
}