Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae
Abstract
A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of /und Streptococcus/ /und pneumoniae/. Plasmid pSM22, the vector containing the pneumococcal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 fig., 1 tab.
- Inventors:
- Issue Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- OSTI Identifier:
- 6805409
- Patent Number(s):
- 6090623
- Assignee:
- TIC; EDB-88-179643
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; GENES; DNA-CLONING; BACILLUS SUBTILIS; DNA POLYMERASES; DNA REPLICATION; DNA SEQUENCING; INVENTIONS; PLASMIDS; STREPTOCOCCUS; TRACER TECHNIQUES; BACILLUS; BACTERIA; CELL CONSTITUENTS; CLONING; ENZYMES; ISOTOPE APPLICATIONS; MICROORGANISMS; NUCLEIC ACID REPLICATION; NUCLEOTIDYLTRANSFERASES; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; STRUCTURAL CHEMICAL ANALYSIS; TRANSFERASES; 550200* - Biochemistry
Citation Formats
Lacks, S A, Martinez, S, Lopez, P, and Espinosa, M. Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae. United States: N. p., 1987.
Web.
Lacks, S A, Martinez, S, Lopez, P, & Espinosa, M. Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae. United States.
Lacks, S A, Martinez, S, Lopez, P, and Espinosa, M. Fri .
"Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae". United States.
@article{osti_6805409,
title = {Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae},
author = {Lacks, S A and Martinez, S and Lopez, P and Espinosa, M},
abstractNote = {A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of /und Streptococcus/ /und pneumoniae/. Plasmid pSM22, the vector containing the pneumococcal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 fig., 1 tab.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Aug 28 00:00:00 EDT 1987},
month = {Fri Aug 28 00:00:00 EDT 1987}
}
