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Title: On-line DNA analysis system with rapid thermal cycling

Abstract

This application describes an apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a chromatography device including an injection means, a chromatography pump, and a chromatography column. In addition, the apparatus also contains a capillary electrophoresis device consisting of a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column. Effluent from the liquid chromatography column passes over the inlet end of the capillary electrophoresis column through a tee structure and when the loading of the capillary electrophoresis column is desired, a voltage supply is activated at a precise voltage and polarity over a specific duration to cause sample species to be diverted from the flowing stream to the capillary electrophoresis column. A laser induced fluorescence detector preferably is used to analyze the products separated while in the electrophoresis column. 6 figs.

Inventors:
;
Issue Date:
Research Org.:
Oak Ridge Associated Universities
Sponsoring Org.:
USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
OSTI Identifier:
678584
Patent Number(s):
5935522
Application Number:
PAN: 8-885,625; CNN: Contract 5P30HG00199
Assignee:
Univ. of Utah Research Foundation, Salt Lake City, UT (United States)
DOE Contract Number:  
AC05-76OR00033
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 10 Aug 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; SAMPLE PREPARATION; CHROMATOGRAPHY; BIOLOGICAL MATERIALS; ELECTROPHORESIS; LASERS; DESIGN

Citation Formats

Swerdlow, H P, and Wittwer, C T. On-line DNA analysis system with rapid thermal cycling. United States: N. p., 1999. Web.
Swerdlow, H P, & Wittwer, C T. On-line DNA analysis system with rapid thermal cycling. United States.
Swerdlow, H P, and Wittwer, C T. Tue . "On-line DNA analysis system with rapid thermal cycling". United States.
@article{osti_678584,
title = {On-line DNA analysis system with rapid thermal cycling},
author = {Swerdlow, H P and Wittwer, C T},
abstractNote = {This application describes an apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a chromatography device including an injection means, a chromatography pump, and a chromatography column. In addition, the apparatus also contains a capillary electrophoresis device consisting of a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column. Effluent from the liquid chromatography column passes over the inlet end of the capillary electrophoresis column through a tee structure and when the loading of the capillary electrophoresis column is desired, a voltage supply is activated at a precise voltage and polarity over a specific duration to cause sample species to be diverted from the flowing stream to the capillary electrophoresis column. A laser induced fluorescence detector preferably is used to analyze the products separated while in the electrophoresis column. 6 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {8}
}