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Title: Method for the enzymatic production of hydrogen

Abstract

The present invention is an enzymatic method for producing hydrogen comprising the steps of: (a) forming a reaction mixture within a reaction vessel comprising a substrate capable of undergoing oxidation within a catabolic reaction, such as glucose, galactose, xylose, mannose, sucrose, lactose, cellulose, xylan and starch; the reaction mixture also comprising an amount of glucose dehydrogenase in an amount sufficient to catalyze the oxidation of the substrate, an amount of hydrogenase sufficient to catalyze an electron-requiring reaction wherein a stoichiometric yield of hydrogen is produced, an amount of pH buffer in an amount sufficient to provide an environment that allows the hydrogenase and the glucose dehydrogenase to retain sufficient activity for the production of hydrogen to occur and also comprising an amount of nicotinamide adenine dinucleotide phosphate sufficient to transfer electrons from the catabolic reaction to the electron-requiring reaction; (b) heating the reaction mixture at a temperature sufficient for glucose dehydrogenase and the hydrogenase to retain sufficient activity and sufficient for the production of hydrogen to occur, and heating for a period of time that continues until the hydrogen is no longer produced by the reaction mixture, wherein the catabolic reaction and the electron-requiring reactions have rates of reaction dependentmore » upon the temperature; and (c) detecting the hydrogen produced from the reaction mixture. 8 figs.« less

Inventors:
;
Issue Date:
Research Org.:
Lockheed Martin Energy Research Corporation
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
678553
Patent Number(s):
5,942,424
Application Number:
PAN: 8-879,243
Assignee:
Lockheed Martin Energy Research Corp., Oak Ridge, TN (United States) PTO; SCA: 080106; PA: EDB-99:083907; SN: 99002124496
DOE Contract Number:  
AC05-96OR22464
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 24 Aug 1999
Country of Publication:
United States
Language:
English
Subject:
08 HYDROGEN FUEL; HYDROGEN PRODUCTION; ENZYMATIC HYDROLYSIS; OXIDOREDUCTASES; HYDROGENASES; ELECTRON TRANSFER; HEXOSES; POLYSACCHARIDES

Citation Formats

Woodward, J., and Mattingly, S.M. Method for the enzymatic production of hydrogen. United States: N. p., 1999. Web.
Woodward, J., & Mattingly, S.M. Method for the enzymatic production of hydrogen. United States.
Woodward, J., and Mattingly, S.M. Tue . "Method for the enzymatic production of hydrogen". United States.
@article{osti_678553,
title = {Method for the enzymatic production of hydrogen},
author = {Woodward, J. and Mattingly, S.M.},
abstractNote = {The present invention is an enzymatic method for producing hydrogen comprising the steps of: (a) forming a reaction mixture within a reaction vessel comprising a substrate capable of undergoing oxidation within a catabolic reaction, such as glucose, galactose, xylose, mannose, sucrose, lactose, cellulose, xylan and starch; the reaction mixture also comprising an amount of glucose dehydrogenase in an amount sufficient to catalyze the oxidation of the substrate, an amount of hydrogenase sufficient to catalyze an electron-requiring reaction wherein a stoichiometric yield of hydrogen is produced, an amount of pH buffer in an amount sufficient to provide an environment that allows the hydrogenase and the glucose dehydrogenase to retain sufficient activity for the production of hydrogen to occur and also comprising an amount of nicotinamide adenine dinucleotide phosphate sufficient to transfer electrons from the catabolic reaction to the electron-requiring reaction; (b) heating the reaction mixture at a temperature sufficient for glucose dehydrogenase and the hydrogenase to retain sufficient activity and sufficient for the production of hydrogen to occur, and heating for a period of time that continues until the hydrogen is no longer produced by the reaction mixture, wherein the catabolic reaction and the electron-requiring reactions have rates of reaction dependent upon the temperature; and (c) detecting the hydrogen produced from the reaction mixture. 8 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {8}
}