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Title: Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages

Abstract

This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. 12 figs.

Inventors:
;
Issue Date:
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
675831
Patent Number(s):
5,824,528
Application Number:
PAN: 8-292,081; CNN: Grant GM21872
Assignee:
Associated Universities, Inc., Washington, DC (United States) PTO; SCA: 550200; 550700; PA: EDB-99:003694; SN: 98002028001
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 20 Oct 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; RNA POLYMERASES; DNA-CLONING; GENE REGULATION; GENES; BACTERIOPHAGES; LYSOZYME

Citation Formats

Studier, F.W., and Dubendorff, J.W. Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages. United States: N. p., 1998. Web.
Studier, F.W., & Dubendorff, J.W. Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages. United States.
Studier, F.W., and Dubendorff, J.W. Tue . "Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages". United States.
@article{osti_675831,
title = {Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages},
author = {Studier, F.W. and Dubendorff, J.W.},
abstractNote = {This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. 12 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {10}
}