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Title: Method for construction of normalized cDNA libraries

Abstract

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries. 19 figs.

Inventors:
;
Issue Date:
Research Org.:
Columbia Univ., New York, NY (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
675824
Patent Number(s):
5830662
Application Number:
PAN: 8-619,542
Assignee:
Columbia Univ., New York, NY (United States)
DOE Contract Number:  
FG02-91ER61233
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 3 Nov 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; DESIGN; DNA; DNA REPLICATION

Citation Formats

Soares, M B, and Efstratiadis, A. Method for construction of normalized cDNA libraries. United States: N. p., 1998. Web.
Soares, M B, & Efstratiadis, A. Method for construction of normalized cDNA libraries. United States.
Soares, M B, and Efstratiadis, A. Tue . "Method for construction of normalized cDNA libraries". United States.
@article{osti_675824,
title = {Method for construction of normalized cDNA libraries},
author = {Soares, M B and Efstratiadis, A},
abstractNote = {This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries. 19 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {11}
}