Method for construction of normalized cDNA libraries
Abstract
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries. 19 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Columbia Univ., New York, NY (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 675824
- Patent Number(s):
- 5830662
- Application Number:
- PAN: 8-619,542
- Assignee:
- Columbia Univ., New York, NY (United States)
- DOE Contract Number:
- FG02-91ER61233
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 3 Nov 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; DESIGN; DNA; DNA REPLICATION
Citation Formats
Soares, M B, and Efstratiadis, A. Method for construction of normalized cDNA libraries. United States: N. p., 1998.
Web.
Soares, M B, & Efstratiadis, A. Method for construction of normalized cDNA libraries. United States.
Soares, M B, and Efstratiadis, A. Tue .
"Method for construction of normalized cDNA libraries". United States.
@article{osti_675824,
title = {Method for construction of normalized cDNA libraries},
author = {Soares, M B and Efstratiadis, A},
abstractNote = {This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries. 19 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {11}
}