Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages
Abstract
This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. 12 figs.
- Inventors:
- Issue Date:
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 675823
- Patent Number(s):
- 5830694
- Application Number:
- PAN: 8-752,495; CNN: Grant GM21872
- Assignee:
- Associated Universities, Inc., Washington, DC (United States)
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 3 Nov 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; GENE REGULATION; RNA POLYMERASES; BACTERIOPHAGES; GENES
Citation Formats
Studier, F W, and Dubendorff, J W. Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages. United States: N. p., 1998.
Web.
Studier, F W, & Dubendorff, J W. Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages. United States.
Studier, F W, and Dubendorff, J W. Tue .
"Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages". United States.
@article{osti_675823,
title = {Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages},
author = {Studier, F W and Dubendorff, J W},
abstractNote = {This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. 12 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {11}
}