Flow cytometric measurement of total DNA and incorporated halodeoxyuridine
Abstract
A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)
- Inventors:
- Issue Date:
- OSTI Identifier:
- 6747531
- Application Number:
- ON: DE84014504
- Assignee:
- Dept. of Energy
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; DEOXYURIDINE; MEASURING METHODS; DNA; ANIMAL CELLS; BIOSYNTHESIS; CELL CYCLE; CELL FLOW SYSTEMS; IN VITRO; IN VIVO; LABELLED COMPOUNDS; MONOCLONAL ANTIBODIES; ORGANIC HALOGEN COMPOUNDS; TRACER TECHNIQUES; ANTIBODIES; ANTIMETABOLITES; AZINES; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RIBOSIDES; SYNTHESIS; URACILS; 550201* - Biochemistry- Tracer Techniques
Citation Formats
Dolbeare, F A, and Gray, J W. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine. United States: N. p., 1983.
Web.
Dolbeare, F A, & Gray, J W. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine. United States.
Dolbeare, F A, and Gray, J W. Tue .
"Flow cytometric measurement of total DNA and incorporated halodeoxyuridine". United States.
@article{osti_6747531,
title = {Flow cytometric measurement of total DNA and incorporated halodeoxyuridine},
author = {Dolbeare, F A and Gray, J W},
abstractNote = {A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 18 00:00:00 EDT 1983},
month = {Tue Oct 18 00:00:00 EDT 1983}
}