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Title: Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

Abstract

A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

Inventors:
;
Issue Date:
OSTI Identifier:
6747531
Application Number:
ON: DE84014504
Assignee:
Dept. of Energy ERA-09-036344; EDB-84-120460
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DEOXYURIDINE; MEASURING METHODS; DNA; ANIMAL CELLS; BIOSYNTHESIS; CELL CYCLE; CELL FLOW SYSTEMS; IN VITRO; IN VIVO; LABELLED COMPOUNDS; MONOCLONAL ANTIBODIES; ORGANIC HALOGEN COMPOUNDS; TRACER TECHNIQUES; ANTIBODIES; ANTIMETABOLITES; AZINES; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RIBOSIDES; SYNTHESIS; URACILS 550201* -- Biochemistry-- Tracer Techniques

Citation Formats

Dolbeare, F.A., and Gray, J.W. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine. United States: N. p., 1983. Web.
Dolbeare, F.A., & Gray, J.W. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine. United States.
Dolbeare, F.A., and Gray, J.W. Tue . "Flow cytometric measurement of total DNA and incorporated halodeoxyuridine". United States.
@article{osti_6747531,
title = {Flow cytometric measurement of total DNA and incorporated halodeoxyuridine},
author = {Dolbeare, F.A. and Gray, J.W.},
abstractNote = {A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1983},
month = {10}
}