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Title: Method for polymer synthesis in a reaction well

Abstract

A method of synthesis is described for building a polymer chain, oligonucleotides in particular, by sequentially adding monomer units to at least one solid support for growing and immobilizing a polymer chain thereon in a liquid reagent solution. The method includes the step of: (A) depositing a liquid reagent in a reaction well in contact with at least one solid support and at least one monomer unit of the polymer chain affixed to the solid support. The well includes at least one orifice extending into the well, and is of a size and dimension to form a capillary liquid seal to retain the reagent solution in the well to enable polymer chain growth on the solid support. The method further includes the step of (B) expelling the reagent solution from the well, while retaining the polymer chain therein. This is accomplished by applying a first gas pressure to the reaction well such that a pressure differential between the first gas pressure and a second gas pressure exerted on an exit of the orifice exceeds a predetermined amount sufficient to overcome the capillary liquid seal and expel the reagent solution from the well through the orifice exit. 9 figs.

Inventors:
Issue Date:
Sponsoring Org.:
USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
OSTI Identifier:
672623
Patent Number(s):
5,814,700
Application Number:
PAN: 8-453,972; CNN: Contract LBL 4437-10;Contract 3POI HG00205-0251
Assignee:
Leland Stanford Junior Univ., Stanford, CA (United States) PTO; SCA: 550200; PA: EDB-98:121623; SN: 98002026809
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 29 Sep 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; OLIGONUCLEOTIDES; SYNTHESIS; REAGENTS; CHEMICAL REACTORS; POLYMERIZATION; SEPARATION PROCESSES

Citation Formats

Brennan, T.M. Method for polymer synthesis in a reaction well. United States: N. p., 1998. Web.
Brennan, T.M. Method for polymer synthesis in a reaction well. United States.
Brennan, T.M. Tue . "Method for polymer synthesis in a reaction well". United States.
@article{osti_672623,
title = {Method for polymer synthesis in a reaction well},
author = {Brennan, T.M.},
abstractNote = {A method of synthesis is described for building a polymer chain, oligonucleotides in particular, by sequentially adding monomer units to at least one solid support for growing and immobilizing a polymer chain thereon in a liquid reagent solution. The method includes the step of: (A) depositing a liquid reagent in a reaction well in contact with at least one solid support and at least one monomer unit of the polymer chain affixed to the solid support. The well includes at least one orifice extending into the well, and is of a size and dimension to form a capillary liquid seal to retain the reagent solution in the well to enable polymer chain growth on the solid support. The method further includes the step of (B) expelling the reagent solution from the well, while retaining the polymer chain therein. This is accomplished by applying a first gas pressure to the reaction well such that a pressure differential between the first gas pressure and a second gas pressure exerted on an exit of the orifice exceeds a predetermined amount sufficient to overcome the capillary liquid seal and expel the reagent solution from the well through the orifice exit. 9 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {9}
}