Surface enhanced Raman gene probe and methods thereof
Abstract
The subject invention disclosed is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Lockheed Martin Energy Syst Inc
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 672560
- Patent Number(s):
- 5,783,389
- Application Number:
- PAN: 8-791,981
- Assignee:
- Lockheed Martin Energy Research Corp., Oak Ridge, TN (United States) PTO; SCA: 550200; PA: EDB-98:121629; SN: 98002024815
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 21 Jul 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; OLIGONUCLEOTIDES; DNA HYBRIDIZATION; RAMAN SPECTROSCOPY; DNA; RNA; FIBER OPTICS
Citation Formats
Vo-Dinh, T. Surface enhanced Raman gene probe and methods thereof. United States: N. p., 1998.
Web.
Vo-Dinh, T. Surface enhanced Raman gene probe and methods thereof. United States.
Vo-Dinh, T. Tue .
"Surface enhanced Raman gene probe and methods thereof". United States.
@article{osti_672560,
title = {Surface enhanced Raman gene probe and methods thereof},
author = {Vo-Dinh, T.},
abstractNote = {The subject invention disclosed is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {7}
}