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Title: Dyes designed for high sensitivity detection of double-stranded DNA

Abstract

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye. 10 figs.

Inventors:
;
Issue Date:
Research Org.:
Univ. of California (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
672559
Patent Number(s):
5783687
Application Number:
PAN: 8-708,666
Assignee:
Univ. of California, Berkeley, CA (United States)
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 21 Jul 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DYES; DNA; AFFINITY; ELECTROPHORESIS; FLUORESCENCE; CHEMICAL BONDS

Citation Formats

Glazer, A N, and Benson, S C. Dyes designed for high sensitivity detection of double-stranded DNA. United States: N. p., 1998. Web.
Glazer, A N, & Benson, S C. Dyes designed for high sensitivity detection of double-stranded DNA. United States.
Glazer, A N, and Benson, S C. Tue . "Dyes designed for high sensitivity detection of double-stranded DNA". United States.
@article{osti_672559,
title = {Dyes designed for high sensitivity detection of double-stranded DNA},
author = {Glazer, A N and Benson, S C},
abstractNote = {Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye. 10 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {7}
}