Dyes designed for high sensitivity detection of double-stranded DNA
Abstract
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye. 10 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 672559
- Patent Number(s):
- 5783687
- Application Number:
- PAN: 8-708,666
- Assignee:
- Univ. of California, Berkeley, CA (United States)
- DOE Contract Number:
- FG03-91ER61125
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 21 Jul 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DYES; DNA; AFFINITY; ELECTROPHORESIS; FLUORESCENCE; CHEMICAL BONDS
Citation Formats
Glazer, A N, and Benson, S C. Dyes designed for high sensitivity detection of double-stranded DNA. United States: N. p., 1998.
Web.
Glazer, A N, & Benson, S C. Dyes designed for high sensitivity detection of double-stranded DNA. United States.
Glazer, A N, and Benson, S C. Tue .
"Dyes designed for high sensitivity detection of double-stranded DNA". United States.
@article{osti_672559,
title = {Dyes designed for high sensitivity detection of double-stranded DNA},
author = {Glazer, A N and Benson, S C},
abstractNote = {Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye. 10 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 21 00:00:00 EDT 1998},
month = {Tue Jul 21 00:00:00 EDT 1998}
}
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